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MetabolomicsGlobal 13C tracing and metabolic flux analysis of intact human liver tissue ex vivo
Liver metabolism is central to human physiology and influences the pathogenesis of common metabolic diseases. Yet, our understanding of human liver metabolism remains incomplete, with much of current knowledge based on animal or cell culture models that do not fully recapitulate human physiology. He...
2024-07-23 | MTBLS10481 | MetaboLights
MetabolomicsElucidation of xenoestrogen metabolism by non-targeted, stable isotope-assisted mass spectrometry in breast cancer cells

Environmental exposure to xenoestrogens, i.e., chemicals that imitate the hormone 17β-estradiol, has the potential to influence hormone homeostasis and action. Detailed knowledge of xenobiotic biotransformation processes in cell models is key when transferring knowledge learned from in vitro<...

2023-10-26 | MTBLS2716 | MetaboLights
ProteomicsLarge scale intact glycopeptide identification by Mascot database search
We present the adaptability of Mascot search engine for automated identification of intact glycopeptide mass spectra. The steps involved in adopting Mascot for intact glycopeptide analysis include: i) assigning unique one letter codes for monosaccharides, ii) linearizing glycan sequences and iii) pr...
ORGANISM(S): Homo sapiens (Human) 
2018-02-08 | PXD005931 | Pride
Mascot Glycoproteomics Higher-energy collisional induced dissociation (hcd) Prostate cancer Serum 
ProteomicsDe Novo Sequencing of Polyclonal Antibodies by Integrating Intact Mass, Top-Down and Bottom-Up Mass Spectrometry
Antibodies are specialized proteins produced by the adaptive immune system to identify and neutralize harmful antigens. Antibody-based diagnostics and therapeutics have advanced rapidly, with recombinant antibodies derived from monoclonal antibodies (mAbs) becoming the preferred standard due to thei...
ORGANISM(S): Homo sapiens (Human) 
2025-10-31 | PXD063526 | Pride
Intact mass De novo sequencing Top-down Bottom-up Polyclonal antibody sequencing 
ProteomicsOptimization of Higher-Energy Collisional Dissociation Fragmentation Energies for Intact Protein-level Tandem Mass Tag Labeling
Isobaric chemical tag labeling (e.g., iTRAQ and TMT) is a commonly used approach in quantitative proteomics research. Typically, peptides are covalently labeled with isobaric chemical tags, and quantification is enabled through detection of low-mass reporter ions generated after MS2 fragmentation. R...
ORGANISM(S): Homo sapiens (Human) 
2023-01-17 | PXD037491 | Pride
Protein-level tmt labeling  top-down proteomics  quantitative proteomics  hcd 
ProteomicsHistone interaction landscapes visualized by crosslinking mass spectrometry in intact cell nuclei
Cells organize their actions partly through tightly controlled protein-protein interactions – collectively termed the interactome. Here we use crosslinking mass spectrometry (XL-MS) to chart the interactome of intact human nuclei. We overall identified ~8700 crosslinks, of which 2/3 represent links ...
ORGANISM(S): Homo sapiens (Human) 
2018-07-23 | PXD007513 | Pride
Crosslinking mass spectrometry Histone interaction network – ptm interplay Nuclear interactome Histone variant specific interactions Nuclear protein-protein interactions 
ProteomicsMALDI-MS Profiling of Intact Blood Proteins without the Need for Freezing - Dried Serum Spots as Future Clinical Tools for Patient Screening
To open new ways for MALDI-MS-based patient screening, blood serum is the most preferred specimen because of its richness in patho-physiological information and due to ease of collection. To overcome deleterious freeze / thaw cycles and to reduce high costs for shipping and storage, we sought to dev...
ORGANISM(S): Homo sapiens (Human) 
2021-05-20 | PXD024669 | Pride
Intact serum protein analysis Maldi-ms profiling Fast frozen serum Serum proteolysis analysis Dried serum spots 
ProteomicsBioinformatics Analysis of Top-Down Mass Spectrometry Data with ProSight Lite
Datafiles for Bioinformatics Analysis of Top-Down Mass Spectrometry Data with ProSight Lite
ORGANISM(S): Equus Caballus (ncbitaxon:9796) Bos Taurus (ncbitaxon:9913) 
2021-04-13 | MSV000087182 | MassIVE
ProteomicsOnline Hydrophilic Interaction Chromatography (HILIC) Enhanced Top-Down Mass Spectrometry Characterization of SARS-CoV-2 Spike Receptor Binding Domain
Online LCMS intact glycoform separation of SARS-CoV-2 spike receptor binding domain proteins expressed in HEK-293 cells from the vendors Sino Biological or RayBiotech. C2 RPLC, CZE, and HILIC separation methods are compared as well as top-down and bottom-up analysis to define RBD glycosylation patte...
ORGANISM(S): Sars Coronavirus (ncbitaxon:227859) Homo Sapiens (ncbitaxon:9606) 
2021-12-21 | MSV000088601 | MassIVE
UnknownDatabase search algorithm for identification of intact cross-links in proteins and peptides using tandem mass spectrometry.
Not available
S-EPMC4141472 | biostudies-literature
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