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MetabolomicsGlobal 13C tracing and metabolic flux analysis of intact human liver tissue ex vivo
Liver metabolism is central to human physiology and influences the pathogenesis of common metabolic diseases. Yet, our understanding of human liver metabolism remains incomplete, with much of current knowledge based on animal or cell culture models that do not fully recapitulate human physiology. He...
2024-07-23 | MTBLS10481 | MetaboLights
ProteomicsGlobal quantification of intact proteins via chemical isotope labeling and mass spectrometry
Although thousands of intact proteins can be feasibly identified in recent years, global quantification of intact proteins is still challenging. Herein, we develop a high-throughput strategy for global intact protein quantification based on chemical isotope labeling. The isotope incorporation effici...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-04-30 | PXD012526 | Pride
Intact protein quantification Ptop Chemical isotope labeling Highest isotopic peak Mass spectrometry 
ProteomicsHuman serum N-linked intact glycopeptides LC-MS/MS
Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled tandem mass spectrometry (LC-MS/MS) in human serum is challenging due to the wide dynamic range of serum protein abundances, the lack of a complete serum N-Glycan database and the existence of non-specifical...
ORGANISM(S): Armoracia rusticana Homo sapiens (Human) Gallus gallus (Chicken) 
2020-04-21 | PXD015622 | Pride
N-linked intact glycopeptide Co-elution Spectral library search Human serum Isotopic distribution 
ProteomicsAcrylamide-based monolith for HILIC-HRMS of intact (glyco)proteins
HILIC-HRMS separations of four intact proteins, such as cytochrome c, carbonic anhydrase, bovine serum albumin, transferrin and separations of five glycoforms of ribonuclease b
ORGANISM(S): Intact Proteins From Several Species 
2021-08-12 | MSV000087977 | MassIVE
ProteomicsGlycosylation Profiling of Neoplastic Biomarker Alpha Fetoprotein Through Intact Mass Protein Analysis
Glycosylation is an important post-translational modification (PTM) mechanism of proteins, that modulates protein function. Measurement of clinically adopted cancer protein biomarkers is commonly performed using affinity-based techniques, which often suffer from poor specificity and inability to dis...
ORGANISM(S): Homo sapiens (Human) 
2023-01-11 | PXD038602 | Pride
Intact proteins Hepatocellular carcinoma 
ProteomicsIntact Peptides from Wine Haze Proteins
Tryptic peptides from wine haze proteins (TLP and CHI) were identified by MS-based peptidomics (top-down proteomics. Their structural visualization and molecular dynamics simulations were used to highlight cleavage spots.
ORGANISM(S): Vitis vinifera (Grape) 
2022-08-04 | PXD024723 | Pride
Intact peptides wine haze proteins 
ProteomicsIntact mass LC-MS analysis of Flu B HA1 and HA2 proteins
To determine the sequences of the Flu HA1 and HA2 proteins, we performed intact mass LC-MS analysis. By reducing disulfide bridges two peaks corresponding to the HA1 and HA2 components were detected. Mass profiles were obtained for both glycosylated and deglycosylated HA proteins.
ORGANISM(S): Influenza A virus (A/Aquatic bird/Hong Kong/M603/98(H11N1)) 
2025-05-06 | PXD046130 | Pride
Intact mass Ha2 Ha1 Flu b 
ProteomicsMALDI-MS Profiling of Intact Blood Proteins without the Need for Freezing - Dried Serum Spots as Future Clinical Tools for Patient Screening
To open new ways for MALDI-MS-based patient screening, blood serum is the most preferred specimen because of its richness in patho-physiological information and due to ease of collection. To overcome deleterious freeze / thaw cycles and to reduce high costs for shipping and storage, we sought to dev...
ORGANISM(S): Homo sapiens (Human) 
2021-05-20 | PXD024669 | Pride
Intact serum protein analysis Maldi-ms profiling Fast frozen serum Serum proteolysis analysis Dried serum spots 
ProteomicsIntact Protein extraction using Liquid extraction surface analysis (LESA)
Development of a new technique of extraction of intact protein on localized region of tissue section using liquide extraction surface analysis.
ORGANISM(S): Rattus norvegicus (Rat) 
2016-03-14 | PXD001925 | Pride
Lesa Intact proteins extraction 
ModelsLai2014 - Hemiconcerted MWC model of intact calmodulin with two targets
Lai2014 - Hemiconcerted MWC model of intact calmodulin with two targets This model is described in the article: Modulation of calmodulin lobes by different targets: an allosteric model with hemiconcerted conformational transitions. Lai M, Brun D, Edelstein SJ, Le Novère N. ...
2024-09-02 | BIOMD0000000574 | BioModels
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