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TranscriptomicsModified iCLIP sequencing of HEK293 cells with antibody PTBP1
For modified iCLIP experiment, 4SU was used for crosslinking as described in published protocol (citation is bellow) and the RNase conditions were optimised to ensure efficient RNase I-dependent fragmentation. In detail, HEK293T cells were grown on 10 cm 2 dishes, incubated for 8 h with 100 M 4SU an...
ORGANISM(S): Homo sapiens 
2016-11-24 | E-MTAB-5027 | biostudies-arrayexpress
MetabolomicsCyclooxygenase-2, Asymmetric Dimethylarginine, and the Cardiovascular Hazard From Nonsteroidal Anti-Inflammatory Drugs (Murine kidney and plasma UPLC-MS/MS assay)

BACKGROUND: Large-scale, placebo-controlled trials established that nonsteroidal anti-inflammatory drugs confer a cardiovascular hazard: this has been attributed to depression of cardioprotective products of cyclooxygenase (COX)-2, especially prostacyclin. An alternative mechanis...

2019-08-22 | MTBLS680 | MetaboLights
ProteomicsRole of TbTim20 in mitochondrial protein import in trypanosomes – TbTim20 CoIPs
Protein import across the mitochondrial inner membrane typically depends on two protein translocases of the inner membrane (TIM) complexes and the membrane potential. The protozoan parasite Trypanosoma brucei, however, has a single, divergent TIM complex. Unlike other trypanosomal TIM subunits, TbTi...
ORGANISM(S): Trypanosoma brucei 
2025-10-27 | PXD061390 | Pride
Trypanosoma brucei Proteomics Affinity purification-mass spectrometry Mitochondrial genome Silac Mitochondrial protein import 
ProteomicsINTEGRATED MULTI-OMICS ANALYSIS OF PBX1 IN MOUSE ADULT NEURAL STEMAND PROGENITOR CELLS IDENTIFIES A TRANSCRIPTIONAL MODULE WITH RELEVANCE TO LEUKEMOGENESIS
Developmental transcription factors act in networks, but how these networks achieve cell- and tissue specificity is still poorly understood. We here explored pre-B-cell leukemia homeobox 1 (PBX1) in adult neurogenesis combining genomic, transcriptomic, and proteomic approaches. ChIP-Seq analysis unc...
ORGANISM(S): Mus musculus (Mouse) 
2024-09-16 | PXD048222 | Pride
Multi-omics Pbx1 Leukemogenesis 
MetabolomicsCyclooxygenase-2, Asymmetric Dimethylarginine, and the Cardiovascular Hazard From Nonsteroidal Anti-Inflammatory Drugs (Murine and human plasma quantitative L-arginine and methylarginines UPLC-MS/MS assay)

BACKGROUND: Large-scale, placebo-controlled trials established that nonsteroidal anti-inflammatory drugs confer a cardiovascular hazard: this has been attributed to depression of cardioprotective products of cyclooxygenase (COX)-2, especially prostacyclin. An alternative mechanis...

2019-08-22 | MTBLS685 | MetaboLights
ProteomicsDefinition of the human mitochondrial TOM interactome reveals TRABD as new interacting protein (TRABD IP data)
The mitochondrial proteome arises from dual genetic origin. Nuclear-encoded proteins need to be transported across or inserted into two distinguished membranes, and the TOM complex represents the main translocase in the outer mitochondrial membrane. Its composition and regulations have been extensiv...
ORGANISM(S): Homo sapiens (Human) 
2025-04-15 | PXD062440 | Pride
Translocase of the outer mitochondrial membrane Ap-ms Mitochondria Protein import Quantitative mass spectrometry Hek293t cells 
ProteomicsDefinition of the human mitochondrial TOM interactome reveals TRABD as new interacting protein (MAPL IP data)
The mitochondrial proteome arises from dual genetic origin. Nuclear-encoded proteins need to be transported across or inserted into two distinguished membranes, and the TOM complex represents the main translocase in the outer mitochondrial membrane. Its composition and regulations have been extensiv...
ORGANISM(S): Homo sapiens (Human) 
2025-04-15 | PXD062420 | Pride
Translocase of the outer mitochondrial membrane Ap-ms Mitochondria Protein import Quantitative mass spectrometry Hek293t cells 
ProteomicsDefinition of the human mitochondrial TOM interactome reveals TRABD as new interacting protein (TOM20 IP data)
The mitochondrial proteome arises from dual genetic origin. Nuclear-encoded proteins need to be transported across or inserted into two distinguished membranes, and the TOM complex represents the main translocase in the outer mitochondrial membrane. Its composition and regulations have been extensiv...
ORGANISM(S): Homo sapiens (Human) 
2025-04-15 | PXD062418 | Pride
Translocase of the outer mitochondrial membrane Ap-ms Mitochondria Protein import Quantitative mass spectrometry Hek293t cells 
ProteomicsAn Msp1-containing complex removes orphaned proteins in the mitochondrial outer membrane of trypanosomes (SILAC-IP data)
The AAA-ATPase Msp1 extracts mislocalized outer membrane proteins and thus contributes to mitochondrial proteostasis. Using pull down experiments, we show that trypanosomal Msp1 localizes to both the glycosome and the mitochondrial outer membrane, where it forms a stable complex with four outer memb...
ORGANISM(S): Trypanosoma brucei 
2023-07-26 | PXD039631 | Pride
Trypanosoma brucei Proteomics Co-immunoprecipitation Silac Mitochondrial quality control 
ProteomicsWidespread Cytoskeletal Engagement of T. brucei KMP11
KMP11 is an essential and highly conserved kinetoplastid protein. Using immunofluorescence, ultrastructure expansion microscopy and pulldown experiments, we show that KMP11 localizations in Trypanosoma brucei include the flagellum attachment zone, the paraflagellar rod, the flagellar connector, the ...
ORGANISM(S): Trypanosoma brucei 
2026-08-17 | PXD075274 | Pride
Trypanosoma brucei Proteomics Affinity purification-mass spectrometry P197 Silac Kmp11 
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