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ProteomicsmTMT: An alternative, non-isobaric, tandem mass tag allowing for precursor-based quantification
Stable isotope labeling of peptides is the basis for numerous mass spectrometry-based quantification strategies. Isobaric tagging and metabolic labeling, namely TMT and SILAC, are among the most widely used techniques for relative protein quantification. Here we report an alternative, precursor-base...
ORGANISM(S): Homo sapiens (Human) 
2019-09-16 | PXD014545 | Pride
Silac Tmt Isobaric Sh-sy5y Svgp12 Metabolic 
ProteomicsNano-scale solid-phase isobaric labeling for multiplexed quantitative phosphoproteomics
We established a workflow for highly sensitive multiplexed quantitative phosphoproteomics using a nano-scale solid-phase tandem mass tag (TMT) labeling reactor. Phosphopeptides were firstly enriched by titanium oxide chromatography and then labeled with isobaric TMT reagents in a StageTip packed wit...
ORGANISM(S): Homo Sapiens (human) 
2021-07-23 | PXD025536 | JPOST Repository
UnknownResin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation.
Not available
S-EPMC8828046 | biostudies-literature
ProteomicsTandem mass tag labeling to characterize muscle-specific proteome change in beef during early postmortem
Bovine longissimus lumborum (LL) and psoas major (PM) muscles biopsy samples were collected from four carcasses (n = 4) at 45 min, 12 h, and 36 h postmortem from a commercial beef processing facility. Proteome profile variation between beef LL and PM during the early postmortem period were evaluated...
ORGANISM(S): Bos taurus (Bovine) 
2020-02-19 | PXD017535 | Pride
Tmt Muscle-to-meat conversion 
Proteomics29-Plex Tandem Mass Tag Mass Spectrometry Enabling Accurate Quantification by Interference Correction
Tandem mass tag (TMT) mass spectrometry is a mainstream isobaric chemical labeling strategy for profiling proteomes. Here we present a 29-plex TMT method to combine the 11-plex and 18-plex labeling strategies. The 29-plex method was examined with a pooled sample composed of 1x, 3x and 10x E. coli pe...
ORGANISM(S): Homo sapiens (Human) 
2023-03-10 | PXD033865 | Pride
Mass spectrometry  liquid chromatography  proteomics  proteome  tandem mass tag  ratio compression  interference  data processing 
ProteomicsOptimization of Higher-Energy Collisional Dissociation Fragmentation Energies for Intact Protein-level Tandem Mass Tag Labeling
Isobaric chemical tag labeling (e.g., iTRAQ and TMT) is a commonly used approach in quantitative proteomics research. Typically, peptides are covalently labeled with isobaric chemical tags, and quantification is enabled through detection of low-mass reporter ions generated after MS2 fragmentation. R...
ORGANISM(S): Homo sapiens (Human) 
2023-01-17 | PXD037491 | Pride
Protein-level tmt labeling  top-down proteomics  quantitative proteomics  hcd 
UnknownQuantitative Top-Down Proteomics in Complex Samples Using Protein-Level Tandem Mass Tag Labeling.
Not available
S-EPMC8323476 | biostudies-literature
UnknownA benchmarking protocol for intact protein-level Tandem Mass Tag (TMT) labeling for quantitative top-down proteomics.
Not available
S-EPMC9587358 | biostudies-literature
UnknownOptimization of Higher-Energy Collisional Dissociation Fragmentation Energy for Intact Protein-Level Tandem Mass Tag Labeling.
Not available
S-EPMC10164041 | biostudies-literature
UnknownIsobaric protein-level labeling strategy for serum glycoprotein quantification analysis by liquid chromatography-tandem mass spectrometry.
Not available
S-EPMC3690282 | biostudies-literature
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