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TranscriptomicsPacBio Iso-Seq transcriptome sequencing data of multiplexed maize cDNA
Full-Length cDNA transcriptome (Iso-Seq) data sequenced on the PacBio Sequel system using 2.1 chemistry. Multiplexed cDNA library of 12 samples (3 tissues x 4 strains). Tissues: root, embryo, endosperm. Strains: B73, Ki11, B73xKi11, Ki11xB73.
ORGANISM(S): Zea mays 
2020-02-25 | E-MTAB-7837 | biostudies-arrayexpress
TranscriptomicsIso-seq seuqencing of leukemic pooled samples
2 samples have been prepared for ISO-seq sequencing. CD34+ blast cells from 5 MDS patients before 5-AZA treatment (GEO531A16, GEO531A13, GEO531A5, GEO531A11, GEO531A3) were pooled to generate one sample and 2 AML non-treated and 2 CMML non-treated cells (GEO531A2, GEO531A9, GEO531A6, GEO531A7) were ...
ORGANISM(S): Homo sapiens 
2019-12-10 | E-MTAB-8195 | biostudies-arrayexpress
TranscriptomicsIso-Seq analysis of Miscanthus sinensis plants
Iso-Seq (PacBio) sequencing was performed to generate a reference transcriptome library of M. sinensis.
ORGANISM(S): Miscanthus sinensis 
2025-11-21 | E-MTAB-15228 | biostudies-arrayexpress
TranscriptomicsSaccharomyces cerevisiae Transcript Isoform mapping
Extensive transcriptional heterogeneity revealed by isoform profiling Application of TIF-Seq (Transcript IsoForm Sequencing) to S.cerevisiae. The method was applied to simultaneously identify the 5' capped mRNA transcription start site and the 3' polyadenylation site in different conditions: WT cell...
ORGANISM(S): Saccharomyces cerevisiae 
2013-05-06 | E-GEOD-39128 | biostudies-arrayexpress
TranscriptomicsIso-Seq analysis of cell suspension culture and plantlets of Hypericum perforatum
Iso-Seq (PacBio) sequencing was performed to generate a reference library of H. perforatum. We generated genome-wide transcriptome data from in vitro cell suspensions and shoot cultures of H. perforatum.
ORGANISM(S): Hypericum perforatum 
2022-12-31 | E-MTAB-11423 | biostudies-arrayexpress
MetabolomicsNutritional quality and genetic differences of five amaranth cultivars revealed by metabolome profiling and whole-genome sequencing (Seed assays)

BACKGROUND: Amaranth (Amaranthus spp.) has high nutritional quality, with edible grain and leaves, and many agronomic advantages, making it a promising part of the solution for global food insecurity. However, we lack comprehensive metabolomic and genome sequence data fo...

2025-02-25 | MTBLS815 | MetaboLights
MetabolomicsNutritional quality and genetic differences of five amaranth cultivars revealed by metabolome profiling and whole-genome sequencing (Leaf assays)

BACKGROUND: Amaranth (Amaranthus spp.) has high nutritional quality, with edible grain and leaves, and many agronomic advantages, making it a promising part of the solution for global food insecurity. However, we lack comprehensive metabolomic and genome sequence data fo...

2025-02-25 | MTBLS3498 | MetaboLights
TranscriptomicsPacBio Iso-seq of Lupinus angustifolius L. leaf and stem transcriptomes
The aim of this experiment was to obtain full length transcript sequences of genes expressed in leaves and stems of narrow-leafed lupin (Lupinus angustifolius L.) using PacBio Iso-Seq. Total RNA was isolated from leaves and stems of five narrow-leafed lupin (Lupinus angustifolius L.) genotypes with ...
ORGANISM(S): Lupinus angustifolius 
2026-06-30 | E-MTAB-16429 | biostudies-arrayexpress
ProteomicsLong-read transcriptome sequencing reveals abundant promoter diversity in distinct molecular subtypes of gastric cancer
Deregulated gene expression is a hallmark of cancer, however most studies to date have analyzed short-read RNA-sequencing data with inherent limitations. Here, we combine PacBio long-read isoform sequencing (Iso-Seq) and Illumina paired-end short read RNA sequencing to comprehensively survey the tra...
ORGANISM(S): Homo sapiens (Human) 
2021-02-02 | PXD023373 | Pride
Isoform sequencing (iso-seq) Alternative promoters Gastric cancer Long-read sequencing 
TranscriptomicsSingle-molecule, full-length transcript isoform sequencing reveals disease-associated RNA isoforms
Alternative splicing generates differing RNA isoforms that govern phenotypic complexity of eukaryotes. Its malfunction underlies many diseases, including cancer and cardiovascular diseases. Comparative analysis of RNA isoforms at the genome-wide scale has been difficult. Here, we established an expe...
ORGANISM(S): Homo sapiens 
2021-06-16 | E-MTAB-7334 | biostudies-arrayexpress
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