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ProteomicsLFQ of carboxymethyl/carboxyethyl lysine peptides from MGO- or GO-treated HeLa cells
HeLa cells (n=2) were treated with cell culture media containing 2 mM MGO or GO for 2 h and lysed in 6 M guanidine-HCl, 100 mM HEPES-NaOH, pH 7.5, 10 mM TCEP, and 40 mM CAA. The lysates were dissolved by heating and sonication, followed by centrifugation at 20,000 x g for 15 min at 4 ºC. The superna...
ORGANISM(S): Homo Sapiens (human) 
2025-05-07 | PXD063706 | JPOST Repository
ProteomicsLFQ of carboxymethyl/carboxyethyl lysine peptides from WT and DJ-1 KO HeLa cells
WT or DJ-1 KO HeLa cells (n=3) were lysed in 500 μL of 6 M guanidine-HCl, 100 mM HEPES-NaOH, pH 7.5, 10 mM TCEP, and 40 mM CAA. The lysates were dissolved by heating and sonication, followed by centrifugation at 20,000 x g for 15 min at 4 ºC. The supernatants were recovered and proteins (1 mg each) ...
ORGANISM(S): Homo Sapiens (human) 
2025-05-07 | PXD063705 | JPOST Repository
ProteomicsLFQ of 3-phosphoglyceroyl lysine peptides from WT and DJ-1 KO HeLa cells
WT or DJ-1 KO HeLa cells (n=3) were lysed in 500 μL of 6 M guanidine-HCl, 100 mM HEPES-NaOH, pH 7.5, 10 mM TCEP, and 40 mM CAA. The lysates were dissolved by heating and sonication, followed by centrifugation at 20,000 x g for 15 min at 4 ºC. The supernatants were recovered and proteins (1 mg each) ...
ORGANISM(S): Homo Sapiens (human) 
2025-05-07 | PXD063699 | JPOST Repository
ProteomicsPRM of UMR106 pY-IP
UMR106 cells were untreated or treated with 5 mM Pi or 100 ng/ml FGF2 for 15 min. The lysates were digested with trypsin, and tyrosine-phosphorylated peptides were enriched by anti-phosphotyrosine antibody. Several selected peptides including FGFR1 with phosphorylated tyrosine residues were measured...
ORGANISM(S): Rattus Rattus (black Rat) 
2019-05-05 | PXD010791 | JPOST Repository
ProteomicsLFQ of UMR106 pY-IP
UMR106 cells were untreated or treated with 5 mM Pi or 100 ng/ml FGF2 for 15 min. The lysates were digested with trypsin, and tyrosine-phosphorylated peptides were enriched by anti-phosphotyrosine antibody and subjected to LC-MS/MS analysis. Raw data were directly analyzed against the SwissProt data...
ORGANISM(S): Rattus Rattus (black Rat) 
2019-05-05 | PXD010790 | JPOST Repository
ProteomicsProteome data on human MDS-L cells
Shotgun mass spectrometric analysis of WT and TOPORS KO MDS-L cells
ORGANISM(S): Homo Sapiens (human) 
2024-03-08 | PXD048275 | JPOST Repository
ProteomicsMS data on ubiquitinated proteins
Mass spectrometric analysis of ubiquitinated proteins in MDS-L cells
ORGANISM(S): Homo Sapiens (human) 
2024-03-08 | PXD044347 | JPOST Repository
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