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ProteomicsA novel interaction site determines binding between AP180 and AP2 in clathrin mediated endocytosis
The early phases of clathrin mediated endocytosis are organized through a highly complex interaction network mediated by clathrin associated sorting proteins (CLASPs) that comprise long intrinsically disordered regions (IDRs). AP180 is a CLASP exclusively expressed in neurons and comprising a long I...
ORGANISM(S): Bos taurus (Bovine) Mus musculus (Mouse) Rattus norvegicus (Rat) 
2024-07-04 | PXD049300 | Pride
Extended ap180:ap2β2 interaction Xl-ms 
ProteomicsA Membrane-Permeable and Immobilized Metal Affinity Chromatography (IMAC)-Enrichable Cross-Linking Reagent to Advance In Vivo Cross-Linking Mass Spectrometry
We developed a novel membrane-permeable and IMAC-enrichable cross-linker tBu-PhoX. Applying our optimized in vivo XL-MS pipeline, we revealed protein interaction landscape of intact human cells.
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2022-01-11 | PXD029034 | Pride
Proteomics Protein-protein interactions Protein structures Mass spectrometry Crosslinking 
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