A Membrane-Permeable and Immobilized Metal Affinity Chromatography (IMAC)-Enrichable Cross-Linking Reagent to Advance In Vivo Cross-Linking Mass Spectrometry

ABSTRACT: We developed a novel membrane-permeable and IMAC-enrichable cross-linker tBu-PhoX. Applying our optimized in vivo XL-MS pipeline, we revealed protein interaction landscape of intact human cells.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Heart, Cell Culture

SUBMITTER: Pin-Lian Jiang  

LAB HEAD: Fan Liu

PROVIDER: PXD029034 | Pride | 2022-01-11

REPOSITORIES: Pride