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Advances in circadian research revealed an intricate relationship between aging and circadian rhythms. However, whether and how the circadian machinery contribute to stem cell aging, especially in primates, remains poorly understood. In this study, we investigated the role of BMAL1, the only non-red...
ORGANISM(S): Homo sapiens (Human) 
2022-03-18 | PXD030570 | Pride

Circulating tumor cells (CTCs) are recognized as direct seeds of metastasis. However, CTC count may not be the 'best' indicator of metastatic risk because their heterogeneity is generally neglected. In this study, we develop a molecular typing system to predict colorectal cancer metastasis potent...

2023-08-21 | MTBLS5028 | MetaboLights
Proteins obtained in Co-IP were subjected to SDS-PAGE gel electrophoresis and Coomassie brilliant blue staining. After decolorization, the SDS-PAGE gel was sent to the mass spectrometry platform of the Institute of Biophysics, Chinese Academy of Sciences for subsequent processing and mass spectromet...
ORGANISM(S): Homo Sapiens 
2023-07-24 | PXD044052 |
This project is aimed to detect the interacting proteins of mitochondria-tageting actin motor Myo19.
ORGANISM(S): Homo sapiens (Human) 
2022-06-09 | PXD033198 | Pride
Single-base resolution DNA methylomes have been accomplished for both Arabidopsis and human cells which have high genome methylation levels by Illumina ultra-high-throughput bisulfite sequencing technology (MethylC-Seq). Here by combining MethylC-Seq and biological replicate strategies we generated ...
ORGANISM(S): Bombyx mori 
Peptides were labeled with TMT reagents based on the manufacturer’s instructions (Thermo Fisher Scientifific). To quantify six samples, each aliquot (100 µg of peptide equivalent) was reacted with one tube of TMT reagent. After the sample was dissolved in 100 µL of 0.05 M tetraethylammonium bromide ...
ORGANISM(S): Mus Musculus 
2020-05-16 | PXD019233 |
Protein N-phosphorylation enrichment is faced with great difficulties due to the intrinsic instability of the N-P bond, which has seriously hindered its biological function unraveling. Current methods for N-phosphopeptides enrichment are challenging to fulfil high selectivity for all types of N-phos...
ORGANISM(S): Homo Sapiens Escherichia Coli Mus Musculus 
2024-12-12 | PXD058797 |
To investigate the proteomic features in lean subjects with NAFLD, and to identify possible plasma proteins that provide potential application for diagnosing of NAFLD in lean individuals. Participants with or without NAFLD were divided into an overweight NAFLD group, an overweight control group, a l...
ORGANISM(S): Homo Sapiens 
2022-07-21 | PXD035514 |
Identification XPO7-interating proteins by ectopically expressed FLAG-tagged XPO7 proteins in HEK293T cells and performed coimmunoprecipitation (co-IP) with FLAG antibody followed by mass spectrometry analysis.
ORGANISM(S): Homo Sapiens 
2022-11-03 | PXD037890 |
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