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MetabolomicsMetabolic Drug Response Phenotyping in Colorectal Cancer Organoids by LC-QTOF-MS
As metabolic rewiring is crucial for cancer cell proliferation, metabolic phenotyping of patient-derived organoids is desirable to identify drug-induced changes and trace metabolic vulnerabilities of tumor subtypes. We established a novel protocol for metabolomic and lipidomic profiling of colorecta...
2021-10-12 | MTBLS2130 | MetaboLights
MetabolomicsLongitudinal multi-omics reveals subset-specific mechanisms underlying irritable bowel syndrome

The gut microbiome has been implicated in multiple human chronic gastrointestinal (GI) disorders. Determining its mechanistic role in disease has been difficult due to apparent disconnects between animal and human studies and lack of an integrated multi-omics view of disease-specific physiologica...

2020-09-08 | MTBLS1396 | MetaboLights
TranscriptomicsHalobacterium NRC-1 General transcription factor perturbation
Halobacterium cells, specified general transcription factor knock-out strains or plasmid expressed 6-His tagged general transcription factor strains, were grown to mid-log phase. RNA extractions were performed using the Stratagene Absolutely RNA Miniprep Kit and RNA quality checked with the Agilent...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-27 | E-GEOD-7713 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen and light perturbation
A 50ml culture of Halobacterium NRC-1 was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clear flask under constant standard incubator illumination, respectively. Low...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-26 | E-GEOD-7729 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen, light and htr1 perturbation
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 with additional in-frame gene deletion of htr1 was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clea...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-26 | E-GEOD-7726 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen, light and kinA2 perturbation
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 with additional in-frame gene deletion of kinA2 was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard cle...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-26 | E-GEOD-7727 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen, light and htr8 perturbation
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 with additional in-frame gene deletion of htr8 was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clea...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-26 | E-GEOD-7728 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen, light and vng2334C perturbation
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 with additional in-frame gene deletion of vng2334c was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard ...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-26 | E-GEOD-7733 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen, light and zim perturbation
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 with additional in-frame gene deletion of zim was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clear...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-26 | E-GEOD-7734 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen, light and htr2 perturbation
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 with additional in-frame gene deletion of htr2 was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clea...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-26 | E-GEOD-7731 | biostudies-arrayexpress
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