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Viral genomes are most vulnerable to cellular defenses at the start of the infection. A family of jumbo phages related to phage ΦKZ, which infects Pseudomonas aeruginosa, assembles a protein-based phage nucleus to protect replicating phage DNA, but how it is protected prior to phage nucleus assembly...
ORGANISM(S): Pseudomonas aeruginosa PAO1-VE2 Pseudomonas phage phiKZ 
2024-11-26 | PXD049415 | Pride
Viral genomes are most vulnerable to cellular defenses at the start of the infection. A family of jumbo phages related to phage ΦKZ, which infects Pseudomonas aeruginosa, assembles a protein-based phage nucleus to protect replicating phage DNA, but how it is protected prior to phage nucleus assembly...
ORGANISM(S): Pseudomonas aeruginosa PAO1-VE2 Pseudomonas phage phiKZ 
2024-11-26 | PXD050275 | Pride
Large-genome bacteriophages (jumbo phages) of the Chimalliviriadae family assemble a nucleus-like compartment bounded by a protein shell that protects the replicating phage genome from host-encoded restriction enzymes and CRISPR/Cas nucleases. While the nuclear shell provides broad protection agains...
ORGANISM(S): Pseudomonas phage PhiPA3 Escherichia phage vB_EcoM_Goslar 
2024-03-25 | PXD045260 | Pride
fRSL1 is a jumbo myovirus stably and lytically infecting the phytopathogenic bacterium Ralstonia solanacearum. In this study, we investigate the infection cycle of fRSL1 and provide a genomic, proteomic and transcriptomic view of this phage. Its 231-kbp genome sequence showed many genes lacking dete...
ORGANISM(S): Ralstonia solanacearum 
ϕXacN1 is a novel jumbo myovirus infecting the causative agent of Asian citrus canker, Xanthomonas citri. Its linear 384,670 bp double-stranded DNA genome encodes 592 predicted protein coding genes and shows 65,875 bp direct terminal repeats (DTRs), so far the longest DTRs among sequence phage genom...
ORGANISM(S): Cellular Organisms 
Jumbo phages ϕRP31/ϕRP12 infecting Ralstonia solanacearum were isolated in Thailand. Virion proteins of ϕRP31 separated by SDS-PAGE were visualized with Coomassie Brilliant Blue. The protein bands were excised from the gel, digested with trypsin, and analyzed by liquid chromatography-tandem mass spe...
ORGANISM(S): Phage 
A phage nucleus-associated RNA-binding protein required for jumbo phage infection
Large-genome bacteriophages (jumbo phages) of the Chimalliviriadae family assemble a nucleus-like compartment bounded by a protein shell that protects the replicating phage genome from host-encoded restriction enzymes and CRISPR/Cas nucleases. While the nuclear shell provides broad protection agains...
ORGANISM(S): Pseudomonas aeruginosa 
2024-03-12 | GSE243675 | GEO
Phages are important drivers of bacterial evolution with therapeutic potential as antimicrobials. However, gaps in our understanding of phages and ability to rapidly engineer them with new genetic cargo hinders progress towards phage-based therapies. To address the lack of unbiased, genome-wide muta...
ORGANISM(S): Prodigiosinella confusarubida 
2026-07-30 | PXD072185 | Pride
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