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ProteomicsGSK-3 Interactomes from APMS and BioID
Interactomes of GSK-3 Isoforms compared by APMS (in HEK293) and BioID (in HEK293 and HeLa cells).
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-12-15 | MSV000090908 | MassIVE
ProteomicsSARS-CoV-2 variants evolve convergent strategies to remodel the host response: Affinity Purification Mass Spectrometry (APMS)
We sought to compare differences in the SARS-CoV-2 virus-human protein interaction networks between mutated (from the SARS-CoV-2 variants of concern) and wild-type viral proteins. Here, we ectopically expressed 2x-strep-tagged constructs encoding mutant or wild-type SARS-CoV-2 viral proteins in HEK2...
ORGANISM(S): Homo sapiens (Human) Severe acute respiratory syndrome coronavirus 2 
2024-02-16 | PXD036798 | Pride
Omics Apms Lung Sars-cov-2 Host response Host Infection Covid-19 Protein-protein interactions 
ProteomicsMS Analysis In vivo treatment of YAP1 SH tagged with phosphatase inhibitors
Investigation of YAP1 interactome plasticity via APMS
ORGANISM(S): Homo sapiens (Human) 
2023-03-04 | PXD032218 | Pride
Phospho Yap1 Apms 
ProteomicsThe GTPase Nog1 co-ordinates assembly, maturation and quality control of distant ribosomal functional centers
Yeast ribosome assembly analysis, using APMS on various 60S tagged subunits (SSF1, RIX1, ARX1, KRE35, ARX1_bud20D, KRE35_DRG1, KRE35_drg-dn, YEP, G223, NOG1)
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-01-06 | PXD011382 | Pride
Ribosome Apms S cerevisiae 60s 
OtherIn situ spatial reconstruction of distinct normal and pathological cell populations within the human adrenal gland
The human adrenal gland consists of concentrically organized functionally distinct regions responsible for hormone production. Dysregulation of adrenocortical cell differentiation alters the proportion and organization of the functional zones of the adrenal cortex leading to disease. Current models ...
ORGANISM(S): Homo sapiens 
2023-11-15 | GSE244084 | GEO
ProteomicsSILAC APMS anlysis of TSSC4 interactome
U5 snRNP is a complex particle essential for RNA splicing. U5 snRNPs undergo an intricate biogenesis that ensures that only a fully mature particle assembles into a splicing competent U4/U6•U5 tri-snRNP and enters the splicing reaction. During splicing, U5 snRNP is substantially rearranged and leave...
ORGANISM(S): Homo sapiens (Human) 
2021-06-11 | PXD024929 | Pride
Apms  silac  immunoprecipitation 
ProteomicsChlamydia trachomatis effector Tri1 interacts with TRAF7 to displace native TRAF7 interactors
Chlamydia trachomatis is the leading cause of bacterial sexually transmitted infections in the US and preventable blindness worldwide. This obligate intracellular pathogen replicates within a membrane-bound inclusion, but how it acquires nutrients from the host while avoiding detection by the innate...
ORGANISM(S): Homo sapiens (Human) Chlamydia trachomatis 
2024-05-05 | PXD049432 | Pride
Chlamydia trachomatis Apms 
ProteomicsSILAC APMS analysis of SHQ1 interactome in HEK-293 cells
The conserved box H/ACA RNPs consist of one box H/ACA RNA and 4 core proteins: Dyskerin, NHP2, NOP10 and GAR1. The assembly of the H/ACA RNPs is a stepwise process which requires several assembly factors: SHQ1, NAF1, the Survival of Motor Neuron complex SMN, and the R2TP complex. It implicates the c...
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD035330 | Pride
Silac Apms 
ProteomicsSILAC APMS analysis of Pih1D3 interactome
To get insights into the function of Pih1D3 protein, we characterized its partners by performing a proteomic analysis in human cells. We fused this domain to GFP and stably expressed it in HeLa cells using site-specific integration with the Flp-In system. Following differential labeling of GFP-Pih1D...
ORGANISM(S): Homo sapiens (Human) 
2018-06-10 | PXD009499 | Pride
Human Ltq-orbitrap Hela cells Apms Silac 
ProteomicsSILAC APMS analysis of CCDC103 interactome
To get insights into the function of CCD103, we characterized its partners by performing a proteomic analysis in human cells. We fused this domain to GFP and stably expressed it in HeLa cells using site-specific integration with the Flp-In system. Following differential labeling of GFP-CCDC103 and c...
ORGANISM(S): Homo sapiens (Human) 
2018-06-08 | PXD009498 | Pride
Human Ltq-orbitrap Hela cells Apms Silac 
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