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ProteomicsUFM1 E3 ligase promotes recycling of 60S ribosomal subunits from the ER
Supporting MS data for paper (doi: 10.1038/s41586-024-07073-0) by DaRosa P.A., Penchev I. et al., titled "UFM1 E3 ligase promotes recycling of 60S ribosomal subunits from the ER". Index of MS supporting files uploaded: Related to Related to Fig. 1b, c (xb01004(UFM1), xb01005(SBP-UFM1): LFQ IP-MS (Un...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-12-22 | MSV000093721 | MassIVE
ProteomicsUFM1 E3 ligase promotes recycling of 60S ribosomal subunits from the ER
Supporting MS data for paper (doi: 10.1038/s41586-024-07073-0) by DaRosa P.A., Penchev I. et al., titled "UFM1 E3 ligase promotes recycling of 60S ribosomal subunits from the ER". Index of MS supporting files uploaded: - Related to Fig. 1a and Extended Data Fig. 1a (AO3435-AO3440: TMT proteomics (Un...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-11-27 | MSV000093510 | MassIVE
GenomicsStreptomyces cavourensis strain:60S
Streptomyces cavourensis strain:60S Genome sequencing
PRJNA1119507 | ENA
ProteomicsCross-Linking Mass Spectrometry (CXMS) of human pre-60S ribosomal particles
Cross-Linking Mass Spectrometry (CXMS) analysis of human pre-60S ribosomal particles.
ORGANISM(S): Homo Sapiens 
PXD019025 |
ProteomicsRbp95 binds 25S rRNA helix H95 and cooperates with the Npa1 complex during early pre-60S particle maturation
Eukaryotic ribosome synthesis involves more than 200 assembly factors, which promote ribosomal RNA (rRNA) processing, modification and folding, and assembly of ribosomal proteins. The formation and maturation of the earliest pre-60S particles requires structural remodeling by the Npa1 complex, but i...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-10-13 | PXD030106 | Pride
Ribosome assembly factor Yeast S. cerevisiae Tandem-affinity purification (tap) Npa1 Pre-60s particles Rbp95 
ProteomicsEffect of Nop53 depletion on the compositional changes during pre-60S maturation
The eukaryotic ribosome biogenesis is a highly orchestrated multistep process that starts at the nucleolus with the transcription of pre-rRNAs 5S and 35S. The latter comprises the mature 18S, 5.8S and 25S rRNAs separated by internal transcribed spacers (ITS1 and ITS2) and externally flanked by the 5...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-09-09 | PXD023383 | Pride
Nop53 Eukaryotic ribosome biogenesis Pre-60s S. cerevisiae 
ProteomicsIdentification of 60S pre-rRNA binding sites of human GNL3L GTPase
We reveal that the GNL3 paralogue GTPase, GNL3L, associates with pre-ribosomes. We map RNA contact sites on GNL3L as well as GNL3L binding sites on pre-rRNAs. Lack of GNL3L impairs synthesis of the 60S rRNAs and expression of GTPase inactive GNL3L causes defects in early steps of pre-rRNA processing...
ORGANISM(S): Homo sapiens (Human) 
2026-03-05 | PXD067606 | Pride
Human Gtpase Gnl3l 60s rrna Rna-protein crosslinking 
GenomicsEmergence of the primordial pre-60S from the 90S pre-ribosome
Our study gives insight into the assembly pathway when nascent 40S and 60S subunits are still connected but begin to separate.
PRJEB48541 | ENA
GenomicsHcr1/eIF3j is a 60S ribosomal subunit recycling accessory factor in vivo
Hcr1/eIF3j is a 60S ribosomal subunit recycling accessory factor in vivo
PRJNA510985 | ENA
ProteomicsThe novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation
The formation of new ribosomes is tightly coordinated with cell growth and proliferation. In eukaryotes, the correct assembly of all ribosomal proteins and RNAs involves an intricate cascade of maturation and rearrangement steps across three cellular compartments: the nucleolus, nucleoplasm, and cyt...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-10-17 | PXD046691 | Pride
Yeast 60s Ribosome biogenesis Lc-msms 
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