Sort   by:  
 Page size 
GenomicsKAP1(TRIM28/TIF1beta) represses differentiation-inducible genes in embryonic stem cells through cooperative binding with PRC1(Ring1b/Rnf2) and derepresses pluripotency-associated genes (ChIP-seq)
Embryonic stem (ES) cells express pluripotency-associated genes and repress differentiation-inducible genes. The activities of these genes are coordinately reversed during differentiation. The changes in the transcriptome upon conditional KAP1 knockout in ES cells overlapped with the changes durin...
ORGANISM(S): Mus musculus 
2014-07-29 | E-GEOD-56628 | biostudies-arrayexpress
GenomicsFunctional analysis of Kap1 genomic recruitment
A current model for the genomic recruitment of Kap1 is via its interaction with KRAB domain-containing zinc finger transcription factors. We have performed ChIP-seq for various mutant KAP1 proteins and shown that this recruitment mechanism mediates binding of KAP1 only to the 3M-CM-"M-BM-^@M-BM-^Y e...
ORGANISM(S): Homo sapiens 
2011-05-24 | E-GEOD-27929 | biostudies-arrayexpress
GenomicsChIP-Seq of HA tagged ZFP57 and KAP1 in mouse ES cells
We have performed ChIP seq analysis to obtain the positions of KAP1 and ZFP57 binding sites in mouse ES cells. By comparing the two lists, we were able to find bona fide sites. ChIP-Seq of HA tagged ZFP57 and KAP1 in mouse ES cells
ORGANISM(S): Mus musculus 
2011-11-07 | E-GEOD-31181 | biostudies-arrayexpress
TranscriptomicsGenome-wide Analysis of KAP1 Binding Suggests Auto-regulation of KRAB-ZNFs
We performed a genome-scale ChIP-chip comparison of two modifications (trimethylation of lysine 9 and trimethylation of lysine 27) of histone H3 in Ntera2 testicular carcinoma cells and in three different anatomical sources of primary human fibroblasts. We found that in each of the cell types the tw...
ORGANISM(S): Homo sapiens 
2007-09-29 | E-GEOD-8667 | biostudies-arrayexpress
GenomicsChIP-seq of ZFP30-HA and KAP1 in mouse 3T3-L1 cells during adipogenic differentiation
We show that a hitherto poorly characterized KRAB domain-containing zinc-finger (ZF) transcription factor, ZFP30, positively regulates adipogenesis. We demonstrate ZFP30’s function in murine in vitro and in vivo models, as well as in human stromal vascular fraction cells. We reveal through mechanist...
ORGANISM(S): Mus musculus 
2019-03-01 | E-MTAB-6817 | biostudies-arrayexpress
ProteomicsRegulatory role of E3 ubiquitin ligase Smurf2 on transcriptional corepressor KAP1
An E3 ubiquitin ligase Smurf2 and transcriptional co-repressor KAP1 have been documented to play crucial roles in similar cellular pathways including cell cycle, DNA damage response, chromatin compaction, senescence and cell death. Smurf2 and KAP1, through regulation of these cellular processes eith...
ORGANISM(S): Homo sapiens (Human) 
2022-04-07 | PXD029642 | Pride
Human Kap1 E3 ubiquitin ligase Ubiquitination Smurf2 
TranscriptomicsKAP1(TRIM28/TIF1beta) represses differentiation-inducible genes in embryonic stem cells through cooperative binding with PRC1(Ring1b/Rnf2) and derepresses pluripotency-associated genes (expression)
Embryonic stem (ES) cells express pluripotency-associated genes and repress differentiation-inducible genes. The activities of these genes are coordinately reversed during differentiation. The changes in the transcriptome upon conditional KAP1 knockout in ES cells overlapped with the changes durin...
ORGANISM(S): Mus musculus 
2014-05-09 | E-GEOD-56626 | biostudies-arrayexpress
TranscriptomicsKRAB/KAP1-microRNA cascade regulates erythropoiesis through the stage-specific control of mitophagy [Seq]
A multilayered transcription regulatory system is unveiled, where protein- and RNA-based repressors are super-imposed in combinatorial fashion to govern the timely triggering of an essential step of erythropoiesis Analyses of transcriptional profiles and chromatin state in KAP1 WT and KO (or KD) ce...
ORGANISM(S): Homo sapiens 
2013-04-03 | E-GEOD-44061 | biostudies-arrayexpress
GenomicsKAP1 regulates gene networks controlling T cell development and activation
This SuperSeries is composed of the following subset Series: GSE34447: Gene expression analysis of wild type and KAP1 KO mouse T cell progenitors GSE38579: ChIP-seq analysis of KAP1 binding and H3K9me3 modifications in mouse T cell progenitors Refer to individual Series
ORGANISM(S): Mus musculus 
2012-08-07 | E-GEOD-38580 | biostudies-arrayexpress
GenomicsChIP-seq analysis of KAP1 and H3K9me3 modifications in mouse spleen B cells
Chromatin remodeling is fundamental for B cell differentiation. Here, we explored the role in this process of KAP1, the cofactor of KRAB-ZFP transcriptional repressors. B lymphoid-specific Kap1 knockout mice displayed reduced numbers of mature B cells, lower steady-state levels of antibodies and acc...
ORGANISM(S): Mus musculus 
2012-05-16 | E-GEOD-36850 | biostudies-arrayexpress
Sort   by:  
 Page size