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TranscriptomicsDisplacement and re-accumulation of centromeric cohesin during transient pre-anaphase centromere splitting
The ring-shaped cohesin complex links sister chromatids until their timely segregation during mitosis. Cohesin is enriched at centromeres, where it provides the cohesive counter-force to bi-polar tension produced by the mitotic spindle. As a consequence of spindle tension, centromeric sequences tran...
ORGANISM(S): Saccharomyces cerevisiae 
2007-09-29 | E-GEOD-8661 | biostudies-arrayexpress
TranscriptomicsTop1 and Top2-mediated replication fork integrity
DNA topoisomerases solve topological problems during chromosome metabolism. We investigated where and when Top1 and Top2 are recruited on replicating chromosomes and how their inactivation affects fork integrity and DNA damage checkpoint activation. We show that, in the context of replicating chroma...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-05 | E-GEOD-8305 | biostudies-arrayexpress
TranscriptomicsPost-replicative formation of cohesion is required for repair and induced by a single DNA break
Sister chromatid cohesion, established during replication by the protein complex Cohesin, is essential for both chromosome segregation and double-strand break (DSB) repair. Normally cohesion formation is strictly limited to the S-phase of the cell cycle, but DSBs can trigger cohesion also after DNA ...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-10 | E-GEOD-8270 | biostudies-arrayexpress
TranscriptomicsEvidence that Loading of Cohesin Onto Chromosomes Involves Opening of Its SMC Hinge
Cohesin is a multisubunit complex that mediates sister-chromatid cohesion. Its Smc1 and Smc3 subunits possess ABC-like ATPases at one end of 50 nm long coiled coils. At the other ends are pseudosymmetrical hinge domains that interact to create V-shaped Smc1/Smc3 heterodimers. N- and C-terminal domai...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-09 | E-GEOD-4827 | biostudies-arrayexpress
TranscriptomicsNutrient-Regulated Antisense and Intragenic RNAs Modulate a Signal Transduction Pathway
The budding yeast Saccharomyces cerevisiae alters its gene expression profile in response to a change in nutrient availability. The PHO-system is a well-studied case in the transcriptional regulation responding to nutritional changes in which a set of genes (PHO genes) is expressed to activate phosp...
ORGANISM(S): Saccharomyces cerevisiae 
2008-12-23 | E-GEOD-13350 | biostudies-arrayexpress
TranscriptomicsChromosomal association of the Smc5/6 complex reveals that it functions in differently regulated pathways
The SMC protein complexes safeguard genomic integrity through their functions in chromosome segregation and repair. The chromosomal localization of the budding yeast Smc5/6 complex here determined reveals that the complex works specifically on the duplicated genome in differently regulated pathways....
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-11 | E-GEOD-4808 | biostudies-arrayexpress
TranscriptomicsGenome-wide localization of ORC and MCM binding sites and identification of replication origins in S. pombe
DNA replication of eukaryotic chromosomes initiates at a number of discrete loci, called replication origins. Distribution and regulation of origins are important for complete duplication of the genome. Here, we determined locations of Orc1 and Mcm6, components of pre-replicative complex (pre-RC),...
ORGANISM(S): Schizosaccharomyces pombe 
2010-06-11 | E-GEOD-6523 | biostudies-arrayexpress
TranscriptomicsDistribution of different proteins related to sister chromatid cohesion during the cell cycle.
This series is a supplementary data set for the manuscript titled "Cohesin relocation from sites of chromosomal loading to places of convergent transcription" All data shown in the paper were registered (30 ChIP-chip and one transcription data). Experimental design: 1. Type of experiment: a. ChIP ...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-09 | E-GEOD-1461 | biostudies-arrayexpress
TranscriptomicsInvolvement of a chromatin remodeling complex in damage tolerance during DNA replication
ATP-dependent chromatin remodeling complexes have been shown to participate in DNA replication in addition to transcription and DNA repair. However, the mechanisms of their involvement in DNA replication remain unclear. Here, we reveal a specific function of the yeast INO80 chromatin remodeling comp...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-20 | E-GEOD-18570 | biostudies-arrayexpress
TranscriptomicsProtein phosphatase 2A protects centromeric sister chromatid cohesion during meiosis I
Segregation of homologous maternal and paternal centromeres to opposite poles during meiosis I depends on post-replicative crossing over between homologous non-sister chromatids, which creates chiasmata and therefore bivalent chromosomes. Destruction of sister chromatid cohesion along chromosome arm...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-11 | E-GEOD-4791 | biostudies-arrayexpress
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