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UnknownReevaluating the role of megalin in renal vitamin D homeostasis using a human cell-derived microphysiological system
Not available
S-EPMC6896899 | biostudies-literature
UnknownEffects of fuel components and combustion particle physicochemical properties on toxicological responses of lung cells.
Not available
S-EPMC5815945 | biostudies-literature
TranscriptomicsTranscription profiling of T. brucei in vivo or in vitro samples, with in vitro samples either inclubated at 4oC or not to investiage the influence of culturing condtions on intracellular transport
The vast majority of molecular studies on T. brucei are carried out using in vitro cultures. Recently, whole-genome microarray analysis indicated significant differences between in vivo and in vitro cultures of the malaria parasite Plasmodium falciparum. Therefore, as an additional investigation int...
ORGANISM(S): Trypanosoma brucei 
2008-06-26 | E-MEXP-1540 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of T. brucei bloodstream forms cultured in different proportions of fetal bovine serum to investigate iron-sensing mechanisms
Bloodstream trypanosomes are sensitive to iron starvation, and upregulate ESAG6/7 transferrin receptor (TfR) mRNA and protein levels when placed in low transferrin or iron-depleted media, and adjust TfR expression to compensate for reduced endocytosis. Both observations suggest a specific transcript...
ORGANISM(S): Trypanosoma brucei 
2008-06-26 | E-MEXP-1530 | biostudies-arrayexpress
TranscriptomicsRNAi knock-down of VSG in T. brucei for 1 or 3 days
As an additional strategy for investigating T. brucei transcriptome responsiveness, the mRNA of a highly important protein, the variant surface glycoprotein (VSG) the major surface protein in the bloodstream stage, was suppressed with RNAi. VSG RNAi results in arrest of cell cycle progression...
ORGANISM(S): Trypanosoma brucei 
2008-06-26 | E-MEXP-1528 | biostudies-arrayexpress
no omics definedTranscription profiling of T. brucei treated with dithiothreitol (DTT) or tunicamycin to invoke the unfolded protein response
Transcriptome responsiveness was further tested by attempts to invoke the unfolded protein repsonse (UPR), a classic ER-based pathway stimulated by the presence of increased levels of unfolded polypeptides. The UPR is mediated via transcriptional responses in both yeast and metazoan cells, and can b...
ORGANISM(S): Trypanosoma brucei 
2008-06-26 | E-MEXP-1533 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of T. brucei bloodstream and procyclic forms to look at differential expression of membrane-trafficking genes
To look at differential expression of membrane-trafficking genes,
we extracted RNA from exponentially growing cultures of T. brucei
bloodstream (BSF) and procyclic (PCF) forms, labelled and competitively
hybridised the cDNA to the microarray. 8 arrays, representing
6 BSF and 5 PCF bi...
ORGANISM(S): Trypanosoma brucei 
2008-06-26 | E-MEXP-1529 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of T. brucei cell lines over-expressing wild type RAB5A or mutant versions of RAB5A or RAB5B locked in the GTP-bound state
As a test of trypanosome transcriptome responsiveness, we analysed the effects of over-expression of the early endosome small GTPase Rab5A in procyclic form T.brucei, which results in significant augmentation of fluid phase and receptor-mediated endocytic activity (Pal et al. 2002).

part 1: 2...
ORGANISM(S): Trypanosoma brucei 
2008-06-26 | E-MEXP-1539 | biostudies-arrayexpress
TranscriptomicsMicroarray analysis of IL-10 stimulated adherent peripheral blood mononuclear cells
The immune mechanisms that control resistance vs. susceptibility to mycobacterial infection in humans were investigated by studying leprosy skin lesions, the site where the battle between the host and the pathogen is joined. Using an integrative genomics approach, we found an inverse correlation be...
ORGANISM(S): Homo sapiens 
2013-03-05 | E-GEOD-43700 | biostudies-arrayexpress
MetabolomicsContribution of fungal microbiome to intestinal physiology, early-life immune development and mucosal inflammation in mice
The gut microbiome consists of a multi-kingdom microbial community. Whilst the role of bacteria as causal contributors governing host physiological development is well established, the role of fungi remains to be determined. Here, we use germ-free mice colonized with defined species of bacteria, fun...
2022-01-14 | MTBLS1679 | MetaboLights
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