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Genomicslocalization of Scc2 between wild type and scc4-m35 cells in S-phase
Wild-type (AM15307) and scc4-m35 (AM15311) cells carrying SCC2-3FLAG were fixed for 2 hr, 15 min following an a-factor arrest. Samples were harvested, anti-HA ChIP was performed and both input and IP samples were sequenced for both strains. ChIP-seq for S. cerevisiae Scc2-3FLAG in S-phase cells in W...
ORGANISM(S): Saccharomyces cerevisiae 
2016-07-21 | E-GEOD-70316 | biostudies-arrayexpress
GenomicsStructural evidence for Scc4-dependent localization of cohesin loading
Purpose: The Goal of this study is to look at 1) Differences in genome wide localization of Scc2 between wild type and scc4-m35 cells in S-phase 2) Differences in genome wide localisation of Scc1 cohesin between wild type and scc4-m35 in metaphase arrested cells. Method: 1) Wild-type (AM15307) and...
ORGANISM(S): Saccharomyces cerevisiae 
2015-06-05 | E-GEOD-68573 | biostudies-arrayexpress
GenomicsCell-autonomous function of LSH ATPase in de novo DNA methylation
Purpose: We aimed to determine whether the expression of either wild-type or catalytically inactive LSH, carrying a single point mutation in its ATP binding site (K237Q), could restore the levels and patterns of DNA methylation in Lsh-/- mouse embryonic fibroblasts (MEFs). Methods: Lsh-/- MEFs were...
ORGANISM(S): Mus musculus 
2016-07-21 | E-GEOD-64384 | biostudies-arrayexpress
ProteomicsSearching NE-enriched proteomics datasets for splice junction peptides
MudPIT datasets for NaOH-extracted and Salt-and-Detergent (SD)-extracted rat nuclear membranes were obtained from skeletal muscle and liver tissue as described in the following references: Wilkie GS, Korfali N, Swanson SK, Malik P, Srsen V, Batrakou DG, de las Heras J, Zuleger N, Kerr AR, Florens L,...
ORGANISM(S): Rattus Norvegicus (ncbitaxon:10116) 
2018-01-18 | MSV000081951 | MassIVE
GenomicsCell Type-Specific DNA Methylation at Intragenic CpG Islands in the Immune System (MAP-Seq and ChIP-Seq data)
Human and mouse genomes contain a similar number of CpG islands (CGIs), which are discrete CpG-rich DNA sequences associated with transcription start sites. In both species, about 50% of all CGIs are remote from annotated promoters, but nevertheless often have promoter-like features. To document the...
ORGANISM(S): Mus musculus 
2011-05-23 | E-GEOD-25688 | biostudies-arrayexpress
ProteomicsThe long noncoding RNA ADIPINT regulates human adipocyte metabolism via pyruvate carboxylase
The pleiotropic function of long noncoding RNAs is well recognized, but their direct role in governing metabolic homeostasis is less understood. Here, we describe a human adipocyte-specific lncRNA, ADIPINT, that regulates pyruvate carboxylase , a pivotal enzyme in energy metabolism. With a novel app...
ORGANISM(S): Homo sapiens (Human) 
2022-06-09 | PXD032769 | Pride
Human adipocyte troops rna-interaction pyruvate carboxylase metabolism 
GenomicsSaccharomyces cerevisiae
localization of Scc2 between wild type and scc4-m35 cells in S-phase
PRJNA288305 | ENA
TranscriptomicsRNA of Small Cell Lung Cancer Circulating Tumour Cells Derived Explants
RNA-seq data characterising small cell lung cancer within circulating tumour cell (CTC) derived explants, generated within immune compromised mice. Both chemo naive and progression models were taken from patients and their drug sensitivity tested within the mouse models. Blood samples were taken fro...
ORGANISM(S): Homo sapiens 
2020-04-26 | E-MTAB-8465 | biostudies-arrayexpress
TranscriptomicsSoluble Guanylate Cyclase Signalling Mediates Etoposide Resistance in Progressing Small Cell Lung Cancer
We exploited six paired pre-treatment and post-chemotherapy circulating tumour cell patient-derived explant (CDX) models from donors with extensive stage SCLC to investigate changes at disease progression after chemotherapy. WES data from the patient germ line and from the biopsy of the solid tumo...
ORGANISM(S): Homo sapiens 
2021-09-15 | E-MTAB-10880 | biostudies-arrayexpress
GenomicsNeuronal MeCP2 is expressed at near histone-octamer levels and globally alters the chromatin state
We report the application of single molecule-based sequencing technology for high-throughput genom-wide mapping of MeCP2 binding and DNA methylation in mouse brain and cerebellum respectively. We find a good correlation between MeCP2 occupancy and methyl-CpG density and depletion of MeCP2 binding at...
ORGANISM(S): Mus musculus 
2010-03-04 | E-GEOD-19786 | biostudies-arrayexpress
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