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Yeast cell growth and treatment:; Yeast strains used were L51 (MATa, ura3-52, leu2-3, 112, his4-519, ade1-100, trp1::HisG, hap1::LEU2) and MHY100 (MATa, ura3-52, leu2-3, 112, his4-519, ade1-100, hem1-delta100). L51 was used for studies of oxygen regulation, and MHY100 was used for studies of heme r...
ORGANISM(S): Saccharomyces cerevisiae 
Long non-coding RNAs (lncRNAs) have important regulatory roles and can function at the level of chromatin. To determine where lncRNAs bind to chromatin, we developed CHART, a hybridization-based technique that specifically enriches endogenous RNAs along with their targets from reversibly-crosslinked...
ORGANISM(S): Drosophila melanogaster 
The Xist long noncoding RNA (lncRNA) is essential for X-chromosome inactivation (XCI), the process by which mammals compensate for unequal numbers of sex chromosomes. During XCI, Xist coats the future inactive X (Xi) and recruits Polycomb Repressive Complex 2 (PRC2) to the X-inactivation center (Xic...
ORGANISM(S): Mus musculus 
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