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We examined 3D chromatin structure in the absence of cohesin (Scc1-AID auxin-inducible degron) and a control line (E14-Tir1) and found that PRC1 core promoter component RING1B was one of the most enriched proteins. Hence, we performed calibrated ChIP-seq experiments on the control (E14-Tir1) and the...
ORGANISM(S): Mus musculus 
The aim of the experiment was to gain a higher resolution for specific regions of interest to complement the Hi-C results. Capture-C experiments were performed on four-inducible degrons for Scc1, Ringb, Ring1b-Scc1 and CTCF, and Tir1 control, always in triplicates (three biological replicates per co...
ORGANISM(S): Mus musculus 
The aim of this study was to develop methods for the extraction of endogenous peptides from the Asian citrus psyllid, vector of the bacteria that cause the major citrus disease Huanglongbing. In addition, the native peptidome of the Asian citrus psyllid is described, with an emphasis on candidate ne...
ORGANISM(S): Diaphorina citri (Asian citrus psyllid) 
2020-03-19 | PXD014801 | Pride
We performed calibrated total RNAseq on mESC (Scc1-AID) for both untreated and treated cells with a 6-hour auxin exposure to assess the effects of cohesin removal on gene expression. For calibration purposes 2x10ˆ6 Drosophila SG4 cells were spiked in.
ORGANISM(S): Mus musculus 
Porphyromonas gingivalis is a gram-negative bacterium that causes destructive chronic periodontitis. In addition, this bacterium is also involved in the development of cardiovascular disease. The aim of this study was to investigate the effects of P. gingivalis infection on gene and protein expressi...
ORGANISM(S): Homo sapiens 
In this experiment, we've examined chromatin conformation differences of E14 mESC against cohesin and Ring1b degrons. We performed a modified in situ Hi-C protocol from (Rao et al., 2014) that can be found in detail at (Díaz et al., 2018). Samples were digested with MboI restriction enzyme. The aim ...
ORGANISM(S): Mus musculus 
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