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Gel based approach : Concanavalin A bound fiber glycoproteins were resolved through SDS PAGE followed by identification of trypsinised gel pieces through Nano-LC MALDI TOF/TOF. Solution based approach: Concanavalin A bound fiber glycoproteins were solution phase digested and fractionated through SC...
ORGANISM(S): Gossypium 
2014-03-18 | PXD000178 | Pride

Bacteria in the gut can modulate the availability and efficacy of therapeutic drugs. Interactions at this level have only recently started to being systematically mapped and the main underlying mechanism proposed is chemical transformation of drugs by microbes (biotransformation). Here, we invest...

2021-07-20 | MTBLS1627 | MetaboLights
Glycoproteins isolated from elongating cotton fiber cells were analyzed using 2D-PAGE followed by MALDI TOF/TOF based protein identification. Protein isoforms were identified using Tandem mass spectrometry. Lectin based Glycopeptide enrichment strategy followed by PNGase catalyzed deglycosylation re...
ORGANISM(S): Gossypium 
2015-11-25 | PXD002849 | Pride
Bacterial pathogens exploit secreted aminopeptidases to modulate host cellular functions. Mycobacterium tuberculosis (Mtb) secretes PepN, a M1 aminopeptidase with N-terminal peptidase and C-terminal ERAP1_C-like domains. In addition to being conserved across Mtb complex, PepNMtb is also homologous (...
ORGANISM(S): Mycobacterium tuberculosis Mycobacterium smegmatis 
2019-07-29 | PXD009164 | Pride
Transcriptome analysis in cotton under drought stress. To study the molecular response of drought stress in cotton under field condition global gene expression analysis was carried out in leaf tissue. Gossypium hirsutum cv. Bikaneri Nerma was used for the gene expression analysis. Cotton plants were...
ORGANISM(S): Gossypium hirsutum 
Transcriptome analysis in cotton during fibre development stages. To study the molecular response of drought stress in cotton under field condition global gene expression analysis was carried out at fibre development stages (0, 5, 10 and 20 dpa/Days post anthesis). Gossypium hirsutum cv. Bikaneri Ne...
ORGANISM(S): Gossypium hirsutum 

Bacteria in the gut can modulate the availability and efficacy of therapeutic drugs. Interactions at this level have only recently started to being systematically mapped and the main underlying mechanism proposed is chemical transformation of drugs by microbes (biotransformation). Here, we invest...

2021-07-20 | MTBLS1792 | MetaboLights
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