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ProteomicsAP-MS analysis of human RIOK1 and USP16
To discover novel factors involved in the final steps of 40S ribosomal subunit biogenesis in human cells, we isolated late cytoplasmic 40S subunit precursors by affinity purification (AP) of the assembly factor RIOK1. Subsequent analysis of their composition by mass spectrometry (MS) led to identifi...
ORGANISM(S): Homo sapiens (Human) 
2020-03-04 | PXD016458 | Pride
Usp16 Ap-ms Riok1 Dda 
ProteomicsAP-MS analysis of human FUBIeS30/FAU WT and non-cleavable G73,4A and G74V mutants
To discover candidate FUBIeS30 proteases, we performed affinity purification from human cell lysates expressing tandem Strep II-hemagglutinin (StHA)-tagged WT or mutant (G73,74A or G74V) FUBIeS30-StHA constructs. Subsequent analysis of the co-purified proteins by mass spectrometry (LC-MS/MS) identif...
ORGANISM(S): Homo sapiens (Human) 
2021-08-05 | PXD021864 | Pride
Fubies30 Fau Ap-ms Dda Dub 
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