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Polycomb Repressive Complex 1 and histone H2A ubiquitination (ubH2A) contribute to embryonic stem cell (ESC) pluripotency by repressing lineage-specific gene expression. However, whether active deubiquitination co-regulates ubH2A levels in ESCs and during differentiation is not known. Here, we repor...
ORGANISM(S): Mus musculus 
To discover novel factors involved in the final steps of 40S ribosomal subunit biogenesis in human cells, we isolated late cytoplasmic 40S subunit precursors by affinity purification (AP) of the assembly factor RIOK1. Subsequent analysis of their composition by mass spectrometry (MS) led to identifi...
ORGANISM(S): Homo sapiens (Human) 
2020-03-04 | PXD016458 | Pride
The ubiquitin-like modifier ISG15 can modulate host and viral proteins to restrict viral and microbial infections, and act as a cytokine. Its expression and conjugation are strongly up-regulated by type I interferons. Here we identify the deubiquitinating enzyme USP16 as an ISG15 cross-reactive prot...
ORGANISM(S): Homo sapiens (Human) 
2023-11-17 | PXD043553 | Pride
Usp16 deletion-induced gene expression in MEFs
CRISPR-Cas9 editing of human histone deubiquitinase gene USP16 in human monocytic leukemia cell line THP-1
Usp16
USP16 knockdown-induced gene expression in NCI-H23 cells
HEK293T cells were transfected with FLag-USP16, and cells were performed with co-immunoprecipitation by Flag antibody or IgG with protein A/G beads.
ORGANISM(S): Homo Sapiens 
LncEPAT-induced by EGFR inhibits USP16-mediated H2A deubiquitination
Analysis of gene expression in USP16 knockdown PC3 prostate cancer cells
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