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ProteomicsOptimized NHS-amine labeling for multiplexed phosphoproteome study.
In this project, we report the detrimental effect of hydroxylamine, a quenching reagent for NHS-amine reaction, on phosphopeptides. The degree of phosphopeptide enrichment was greatly improved by a desalting method compared with a vacuum-drying method right after quenching reaction. We demonstrate t...
ORGANISM(S): Homo sapiens (Human) 
2018-02-27 | PXD008082 | Pride
Phosphoproteome Β-elimination Hydroxylamine Phosphorylation Tandem mass tag N-hydroxysuccinamide-esters Isobaric tag 
ProteomicsPhosphoproteome profiling using the isobaric carrier without the need for phosphoenrichment
Phosphorylation is a crucial component of signaling cascades in a cell. It controls various biological cellular functions such as cell growth and apoptosis. Due to the low stoichiometry of phosphorylaion, enrichment of phosphopeptide prior to LC-MS/MS is necessary for comprehensive phosphoproteome a...
ORGANISM(S): Homo sapiens (Human) 
2022-03-07 | PXD028490 | Pride
Isobaric labeling Tmt Carrier Phosphoproteome Quantitative proteomics 
ProteomicsMulti-layered proteomic analysis for colorectal cancer
Matrix metalloproteinase-2 (MMP-2) plays a role in cell migration and invasion that involve degradation of extracellular matrix proteins as well as vascular remodeling. Increased expression of MMP-2 has often been observed in the tumors of colorectal cancer (CRC) patients. In order to unveil the per...
ORGANISM(S): Homo sapiens (Human) 
2021-08-26 | PXD018304 | Pride
Mmp-2 Silac Plasma Label-free Focal adhesion kinase Secretome Colorectal cancer 
ProteomicsCommon Repository of FBS Proteins (cRFP) to be added to a search database for mass spectrometric analysis of cell secretome
We propose to use cRFP (common Repository of FBS Proteins) in the MS (mass spectrometry) raw data search of cell secretome. cRFP is a small supplementary sequence list of high abundant fetal bovine serum proteins added to the reference database in use. The aim behind using cRFP is to prevent the con...
ORGANISM(S): Homo sapiens (Human) 
2019-09-04 | PXD015143 | Pride
Common repository of fbs proteins Database search Secretome 
ProteomicsOne-STAGE Tip Method for TMT based Proteomic Analysis of Minimal Amount of Cells
LC-MS-based profiling of proteomes with isobaric tag labeling from low-quantity biological and clinical samples, including needle-core biopsies and laser capture microdissection, has been challenging due to limited amount and sample loss during preparation. To address this problem, we developed OnM ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-04-18 | MSV000091734 | MassIVE
ProteomicsHanging-droplet sample preparation improves coverage for spatially-resolved proteomics
We developed a hanging drop (HD) method to improve the sample recovery for spatial proteomics by placing the nanowell chip in an upside-down direction during droplet-based sample preparation. Compared with the commonly-used sitting-drop (SD) method, the HD method can keep the tissue voxel away from ...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2022-04-21 | MSV000089295 | MassIVE
ProteomicsA comprehensive evaluation of common quantification strategies for LC-MS/MS-based spatial proteomics using nanoPOTS
A deep understanding of the tissue microenvironment can provide significant insight into the function of tissue and organs in health and disease states. Spatially resolved proteomics techniques can aid in studying spatial tissue organization and cellular communication in their native microenvironmen...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-03-22 | MSV000091531 | MassIVE
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