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ProteomicsTi4+-IMAC label-free quantification
We combine high-resolution mass spectrometry with Ti4+-IMAC phosphopeptide enrichment and label-free quantification to monitor the phosphoproteome of Jurkat T-cells following stimulation by Prostaglandin E2. Jurkat T lymphoma cells were grown in RPMI 1640 medium supplemented with 10% fetal bovine se...
ORGANISM(S): Homo sapiens (Human) 
2014-05-22 | PXD000293 | Pride
Label-free phosphoproteomics 
ProteomicsOptimization of label-free nano LC-MS/MS analysis of the placental proteome
We optimized a protocol to analyze the placental proteome by means of label-free nano-LC-MS/MS mass spectrometry with regard to sample treatment, protein extraction, and protein digestion, in order to obtain a high protein concentration for identification of a specific protein signature according to...
ORGANISM(S): Homo sapiens (Human) 
2020-10-13 | PXD020438 | Pride
Placenta Lc-msms 
ProteomicsHuman hippocampus LC-MSMS
Large-scale analysis of post-translational modification in the hippocampus of patients with Alzheimer's disease using pI shift and label free quantification without enrichment.
ORGANISM(S): Homo sapiens (Human) 
2022-02-22 | PXD000950 | Pride
Human brain hippocampus lc-msms 
OtherA Combined Shotgun and Targeted Mass Spectrometry Strategy for Breast Cancer Biomarker Discovery.
Data from ProteomeXchange, PXD ID: PXD001685. File: B08-07165_c.mzml. Published as part of J Proteome Res. 2015 Jun 5 . From the Abstract: {{i} ... Eighty breast tumors, stratified for estrogen receptor status and development of distant recurrence (DR - ), were collected. After enrichment of N-glyc...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
GPM32320015681 | GPMDB
Resource Reanalysis 
ProteomicsBACE1 KO mouse CSF LC-MSMS - Label-free Quantitative Proteomics of Mouse Cerebrospinal Fluid Detects β-Site APP Cleaving Enzyme (BACE1) Protease Substrates In Vivo
Analysis of murine cerebrospinal fluid (CSF) by quantitative mass spectrometry is challenging due to low CSF volume, low total protein concentration and the presence of highly abundant proteins such as albumin. We demonstrate that the CSF proteome of individual mice can be analyzed in a quantitative...
ORGANISM(S): Mus musculus (Mouse) 
2015-10-12 | PXD001514 | Pride
Mouse csf Csf Label-free quantification 
Proteomicsrabbit spleen LC-MSMS label free proteomics studies
9 rabbit spleen label free proteomics studies.In order to study some drugs on animal innate immunity, we built rabbit models, using rabbit immune organs - spleen made mass spectrometry based label free proteomics studies. Finally, it is expected to find the target of the drug and the mechanism of a...
ORGANISM(S): Oryctolagus cuniculus (Rabbit) 
2022-08-12 | PXD029947 | Pride
Rabbit spleen lc-mama 
ProteomicsPea drought tolerance analysis by LC-MSMS
Abiotic stresses caused by adverse environmental conditions are responsible for heavy economic losses on pea crop, being drought one of the most important abiotic constraints. Development of pea cultivars well adapted to dry conditions has been one of the major tasks in breeding programs. The increa...
ORGANISM(S): Pisum sativum (Garden pea) 
2016-08-24 | PXD004587 | Pride
Shotgun Pea Drought tolerance 
ProteomicsLabel-free quantitative proteomics analysis of bone marrow cells of MK-LCM WT versus KO mice
High ploidy large cytoplasmic megakaryocytes (LCM) are critical negative regulators of hematopoietic stem cells (HSC) and are responsible for platelet formation. Using a mouse knockout model with normal megakaryocyte numbers but essentially devoid of LCM (MK-LCM KO), we demonstrated a pronounced inc...
ORGANISM(S): Mus musculus (Mouse) 
2023-05-10 | PXD040423 | Pride
Lc-msms Bone marrow Mouse Lfq 
ProteomicsValidation of proteins showing significant dysregulation from shotgun proteomics experiments in longitudinal plasma samples of COVID-19 patients
A Label Free Quantification study was done to observe the changes in the plasma proteome of patient samples collected at two different time points of disease severity (T1 and T2). A few of the proteins that were significantly dysregulated between the two time points were taken forward for validation...
ORGANISM(S): Homo Sapiens 
2022-06-27 | PXD026848 | panorama
ProteomicsPseudomonas aeruginosa culture supernatant LC-MSMS
In order to investigate the compositions and properties of these protein which we anticipated was crucial to explore their specific functions related with alkane transport and degration, label-free LC-MS/MS strategy was applied for relative quantification of secreted proteins of a n-tetracosane tole...
ORGANISM(S): Pseudomonas aeruginosa SJTD-1 
2023-12-16 | PXD014638 | Pride
Pseudomonas aeruginosa sjtd-1 Alkane Exoproteome 
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