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MetabolomicsAutomated Label-free Quantification of Metabolites from Liquid Chromatography–Mass Spectrometry Data (Plasma)

Liquid chromatography coupled to mass spectrometry (LC-MS) has become a standard technology in metabolomics. In particular, label-free quantification based on LC-MS is easily amenable to large-scale studies and thus well suited to clinical metabolomics. Large-scale studies, however, require autom...

2015-12-16 | MTBLS234 | MetaboLights
MetabolomicsAutomated Label-free Quantification of Metabolites from Liquid Chromatography–Mass Spectrometry Data (Simulated)

Liquid chromatography coupled to mass spectrometry (LC-MS) has become a standard technology in metabolomics. In particular, label-free quantification based on LC-MS is easily amenable to large-scale studies and thus well suited to clinical metabolomics. Large-scale studies, however, require autom...

2015-12-16 | MTBLS235 | MetaboLights
ProteomicsLFQbench enables a multi-centered benchmark study demonstrating robust proteomic label-free quantification
The consistent and accurate quantification of proteins is a challenging task for mass spectrometry (MS)-based proteomics. SWATH-MS uses data-independent acquisition (DIA) for label-free quantification. Here we evaluated five software tools for processing SWATH-MS data: OpenSWATH, SWATH2.0, Skyline, ...
ORGANISM(S): Escherichia Coli (ncbitaxon:562) Homo Sapiens (ncbitaxon:9606) Saccharomyces Cerevisiae (ncbitaxon:4932) 
2017-04-27 | MSV000081024 | MassIVE
ProteomicsSurfactant-assisted one-pot sample preparation for label-free single-cell proteomics
Large numbers of cells are generally required for quantitative global proteome profiling due to the significant surface adsorption losses associated with sample processing. Such bulk measurement obscures important cell-to-cell variability (cell heterogeneity) and makes proteomic profiling impossible...
ORGANISM(S): Homo Sapiens 
2021-03-11 | PXD022827 | panorama
ProteomicsBlack pepper proteome - Transcriptome- Assisted Label-Free Quantitative Proteomics Analysis Reveals Novel Insights into Piper nigrum-Phytophthora capsici Phytopathosystem
In this study, we have evaluated the proteomic changes that occur in Piper nigrum L.(black pepper) after infection by the pathogen Phytophthora capsici. We report novel leaf proteins from black pepper identified by an integrated transcriptome-assisted label-free quantitative proteomics pipeline. Sev...
ORGANISM(S): Piper nigrum 
2016-07-07 | PXD003887 | Pride
Plgs Label-free quantitative proteomics Piper nigrum 
UnknownNovel MMP-9 substrates in cancer cells revealed by a label-free quantitative proteomics approach.
Not available
S-EPMC2577209 | biostudies-literature
ProteomicsAn Integrated Quantitative Proteomics Workflow for Biomarker Discovery and Validation in Plasma
Blood plasma is one of the most widely used samples for biomarker discovery research as well as clinical investigations for diagnostic and therapeutic purposes. However, the plasma proteome is extremely complex due to its wide dynamic range of protein concentrations and the presence of high-abundanc...
ORGANISM(S): Homo sapiens (Human) 
2020-09-07 | PXD017834 | Pride
Targeted proteomics Label-based quantitation Label-free quantitation 
UnknownMetaprotein expression modeling for label-free quantitative proteomics.
Not available
S-EPMC3436780 | biostudies-literature
ProteomicsLabel-free quantitative proteomics in Candida yeast species: technical and biological replicates to asses data reproducibility
Objective Label-free quantitative proteomics has emerged as a powerful strategy to obtain high quality quantitative measures of the proteome with only a very small quantity of total protein extract. Because our research projects were requiring the application of bottom-up shotgun Mass Spectrometry p...
ORGANISM(S): Candida albicans (Yeast) Candida glabrata (Yeast) (Torulopsis glabrata) 
2019-07-08 | PXD014125 | Pride
Mass spectrometry  label-free quantitative proteomics  candida glabrata  candida albicans  alkaline ph 
UnknownTranscriptome- Assisted Label-Free Quantitative Proteomics Analysis Reveals Novel Insights into Piper nigrum-Phytophthora capsici Phytopathosystem.
Not available
S-EPMC4913111 | biostudies-literature
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