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MetabolomicsA combined direct infusion/reversed phase chromatography – high resolution mass spectrometry workflow for accurate absolute lipid quantification with 13C- internal standards
We propose a fully automated novel workflow for lipidomics based on flow injection, followed by liquid chromatography-high-resolution mass spectrometry (FI/LC-HRMS). The workflow combined in-depth characterization of the lipidome achieved via reversed-phase LC-HRMS with absolute quantification by us...
2021-07-01 | MTBLS1876 | MetaboLights
UnknownImproved isobaric tandem mass tag quantification by ion mobility mass spectrometry.
Not available
S-EPMC4000571 | biostudies-literature
ProteomicsQuantitative proteomic analysis of CKD urine samples
Urinary proteomics studies have primarily focused on identifying markers of chronic kidney disease (CKD) progression. Here, we aimed to specify CKD-related injury markers through proteomics analysis in urine of patients with CKD. Label-free quantitative proteomics analysis based on liquid chromatogr...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-06-15 | MSV000087635 | MassIVE
UnknownA quantitative analysis software tool for mass spectrometry-based proteomics.
Not available
S-EPMC3509211 | biostudies-literature
ProteomicsLabel-free LC-MS/MS analysis of silky fowl egg yolk
The proteomic profiles of silky fowl egg yolk (SFEY) and Leghorn egg yolk (LEY) were analysed by bottom-up label-free liquid chromatography tandem-mass spectrometry (LC-MS/MS), aiming to provide a theoretical basis for understanding the proteomic and biological differences between the two yolks and ...
ORGANISM(S): Gallus gallus (Chicken) 
2022-05-20 | PXD032674 | Pride
Label-free proteomic analysis Silky fowl (black-bone chicken) Egg yolk 
ProteomicsReproducibility of label-free single-shot phosphoproteomics applied to CRC cell lines
There is a need for robust phosphopeptide enrichment methods to allow signaling network analysis in cancer cell lines and tissues with minimal fractionation. With recent instrument developments thousands of unique phosphopeptides can be detected by single-shot LC-MS/MS. However, successful phosphopr...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-28 | MSV000080715 | MassIVE
ProteomicsAutomated capillary isoelectric focusing-tandem mass spectrometry for top-down proteomics
Mass spectrometry (MS)-based top-down proteomics (TDP) delineates proteoforms in cells and requires high-resolution proteoform separation. Herein, for the first time, we employed an automated capillary isoelectric focusing-tandem MS (cIEF-MS/MS) system for large-scale TDP of complex proteomes. Singl...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) Escherichia coli 
2020-12-08 | PXD020342 | Pride
Endogenous hormone proteoforms/gel-free 2d-page/multi-level proteomics/ sexual dimorphism/zebrafish brain 
ProteomicsIsolation, enrichment and identification of p62 condensates components by GFP-Trap Agarose Beads pulldown followed by Label Free and Tandem Mass Tag (TMT) mass spectrometry analysis from Hap1 cells
Selective autophagy contributes to the removal of harmful proteins from the cytoplasm. This cargo material is selected by cargo receptors such as p62/SQSTM1 and finally degraded. However, the molecular composition of the p62 condensates and therefore the nature of the cargo delivered by them is inco...
ORGANISM(S): Homo sapiens (Human) 
2023-11-22 | PXD039661 | Pride
Autophagy  cargo receptor  sqstm1  translation 
UnknownComprehensive characterization of glioblastoma tumor tissues for biomarker identification using mass spectrometry-based label-free quantitative proteomics.
Not available
S-EPMC4587597 | biostudies-literature
ProteomicsIdentification of novel blood biomarkers of treatment response in cystic fibrosis pulmonary exacerbations by label-free quantitative proteomics
Pulmonary exacerbations (PEx) are clinically impactful events for individuals with CF. Unfortunately, many CF individuals with PEx fail to regain their baseline lung function despite treatment. The objective of this study was to use unbiased proteomic technology (LC-MS/MS) to identify novel blood pr...
ORGANISM(S): Homo sapiens (Human) 
2020-05-27 | PXD016089 | Pride
Human Cystic fibrosis Plasma Blood Pulmonary exacerbations Lc-ms/ms 
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