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MetabolomicsStable isotope labelling of Toxoplasma gondii under iron and copper restriction

Toxoplasma uses metal nutrients like iron and copper for vital functions in their energy metabolism. Here, we have used stable isotope labelling to track the incorporation of glucose and glutamine into metabolic pathways, primarily focusing on central carbon metabolism, when these metals are disr...

2026-03-18 | MTBLS13492 | MetaboLights
Metabolomics13C glucose labelling studies using 2D NMR is a useful tool for determining ex vivo whole organ metabolism during hypothermic machine perfusion of kidneys
Background: The aim of this study is to determine the feasibility of using Nuclear Magnetic Resonance (NMR) tracer studies (13C-enriched glucose) to detect ex vivo de novo metabolism in the perfusion fluid and cortical tissue of porcine kidneys during hypothermic machine perfusion (HMP). Methods: Po...
2017-10-16 | MTBLS367 | MetaboLights
ModelsNAD labelling dynamics
Model constructed to resample stable isotope labelling experiments using 13C-Glucose
2024-12-23 | MODEL2409150001 | BioModels
Proteomicsyeast membrane proteomics using Leu3 labelling
The yeast signal peptide peptidase homologue Ypf1 functionally interacts with the ER-associated degradation (ERAD) factors Dfm1 and Doa10 to control the abundance of the zinc transporter Zrt1. Here we report that Ctr1 is another substrate for this degradation mechanism with deletion of Ypf1 leading ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2016-04-12 | PXD003052 | Pride
Proteomics Erad Leu3 labelling Yeast Membrane 
ProteomicsEvaluation of NHS-Acetate and DEPC labelling for determination of solvent accessible amino acid residues in protein complexes
The structural analysis of proteins and protein complexes is key for understanding their function and regulation. In order to gain structural information, we examined protein surface labelling using NHS-Acetate and DEPC following an MS-based workflow. Using two model proteins we characterise the obt...
ORGANISM(S): Oryctolagus cuniculus cuniculus Saccharomyces cerevisiae (Baker's yeast) 
2020-05-05 | PXD015940 | Pride
Diethylpyrocarbonate N-hydroxysuccinimidyl acetate Mass spectrometry Neutral loss Chemical labelling Solvent accessible surface area Labelling percentage 
GenomicsiTag labelling of Liver, Plasma and VsWAT RNA
iTag labelling of Liver, Plasma and VsWAT RNA
PRJNA600955 | ENA
GenomicsiTag labelling of Liver, Plasma and VsWAT RNA
iTag labelling of Liver, Plasma and VsWAT RNA
PRJNA551493 | ENA
ProteomicsPlant SILAC: Stable-Isotope Labelling with Amino Acids of Arabidopsis Seedlings for Quantitative Proteomics.
Stable Isotope Labelling by Amino acids in Cell culture (SILAC) is a powerful technique for comparative quantitative proteomics, which has recently been applied to a number of different eukaryotic organisms. Inefficient incorporation of labelled amino acids in cell cultures of Arabidopsis thaliana h...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2016-08-15 | MSV000080043 | MassIVE
TranscriptomicsTranscription profiling of Arabidopsis thaliana seedlings using different Affymetrix RNA labelling protocols
Here we compare transcriptomic data generated using Affymetrix one-cycle (standard labelling protocol), two-cycle (small-sample protocol) and IVT-Express protocols with the Affymetrix ATH1 array using Arabidopsis root samples. Results obtained with each protocol are broadly similar. However, we show...
ORGANISM(S): Arabidopsis thaliana 
2010-03-13 | E-MEXP-2608 | biostudies-arrayexpress
MetabolomicsMass spectrometry imaging of phosphatidylcholine metabolism in lungs administered with therapeutic surfactants and isotopic tracers

Mass spectrometry imaging (MSI) visualises molecular distributions throughout tissues but is blind to dynamic metabolic processes. Here, MSI with high mass resolution together with multiple stable isotope labelling provided spatial analyses of phosphatidylcholine (PC) metabolism in mouse lungs. D...

2020-10-30 | MTBLS2075 | MetaboLights
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