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ProteomicsElectron Transfer/ Higher Energy Collisional Dissociation of Doubly Charged Peptide Ions: Identification of Labile Protein Phosphorylations
Fragmentation behaviour of labile phosphorylated peptides
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2019-05-27 | PXD012989 | Pride
Labile phosphorylations Ethcd 
UnknownSelective Enrichment of Histidine Phosphorylated Peptides Using Molecularly Imprinted Polymers.
Not available
S-EPMC8023515 | biostudies-literature
UnknownDeciphering modifications in swine cardiac troponin I by top-down high-resolution tandem mass spectrometry.
Not available
S-EPMC3056346 | biostudies-literature
UnknownPrecise Readout of MEK1 Proteoforms upon MAPK Pathway Modulation by Individual Ion Mass Spectrometry.
Not available
S-EPMC11008683 | biostudies-literature
UnknownThe Impact of Phosphorylation on Electron Capture Dissociation of Proteins: A Top-Down Perspective.
Not available
S-EPMC5711594 | biostudies-literature
ProteomicsFixated rat liver tissue - Cleavable crosslinkers as tissue fixation reagents for proteomic analysis
Formaldehyde fixation is widely used for long-term maintenance of tissue. However, due to formaldehyde-induced cross-links, fixated tissue proteins are difficult to extract hampering the performance of mass spectrometry (MS)-proteomic analysis on formaldehyde-fixated tissue. Recent years saw the use...
ORGANISM(S): Rattus norvegicus (Rat) 
2018-01-29 | PXD002169 | Pride
Rat liver Tissue fixation Cleavable crosslinkers Reversible crosslinking 
ProteomicsChasing protein arginine phosphorylation: development of a selective enrichment method using a trapping mutant phosphatase
Proof-of-principle pull-down experiments using a YwlE trapping mutant phosphatase to selective capture arginine phosphorylated proteins. Phosphoarginine is an acid labile protein modification that was demonstrated to exist in bacteria and probably also in higher eukaryotes. Due to the high abundance...
ORGANISM(S): Bacteria Bacillus subtilis subsp. subtilis str. 168 
2014-05-19 | PXD000560 | Pride
Arginie phosphorylation Trapping mutant phosphatase 
TranscriptomicsPromoter proximal pausing in response to stress in serum deprived mouse fibroblasts.
We have employed short-capped RNA sequencing (sc-RNA-seq) in order to identify genes whose expression is regulated by promoter proximal pausing of RNA Polymerase II (RNAPI) in response to stress stimulation. We used serum-deprived mouse Swiss 3T3 fibroblasts, either untreated (control) or treated wi...
ORGANISM(S): Mus musculus 
2014-08-12 | E-GEOD-55775 | biostudies-arrayexpress
TranscriptomicsThe epithelial cell transcriptome after alpha-toxin treatment
Responsiveness of cells to alpha-toxin (Hla) from Staphylococcus aureus appears to occur in a cell-type dependent manner. Here, we compare two human bronchial epithelial cell lines, i.e. Hla-susceptible 16HBE14o- and Hla-resistant S9 cells, by a quantitative multi-omics strategy for a better underst...
ORGANISM(S): Homo sapiens 
2015-03-31 | E-GEOD-65018 | biostudies-arrayexpress
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