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TranscriptomicsS.coelicolor_Rok7B7vsM145_MM+mannitol_time_course_expression
For transcript analysis, total RNA was isolated as described (Noens et al, 2007) from surface-grown mycelia of S. coelicolor M145 and its rok7B7 null mutant, cultivated on MM agar plates with mannitol (1% w/v) as the sole carbon source (plates were covered with cellophane discs). Two independent bio...
ORGANISM(S): Streptomyces coelicolor A3(2) 
2014-01-14 | E-MTAB-1465 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Streptomyces coelicolor M600 bldA deletion mutant vs. wild-type M600 S.coelicolor A3(2) time series
Time-dependent gene expression patterns during development and antibiotic production on solid growth medium (SMM) comparing a M600 bldA deletion mutant with wild-type M600 of S.coelicolor A3(2). Eight time points matched in all three replicates of both wild-type and mutant were picked to span the en...
ORGANISM(S): Streptomyces coelicolor 
2008-06-05 | E-MAXD-27 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Streptomyces coelicolor wild type and phP-null mutants at different time points during phosphate starvation
S. coelicolor was grown in low-phosphate concentration R5 liquid medium which facilitated the transition to a phosphate starvation induced stationary phase and the subsequent induction of the PhoP regulon. Global gene expression profiling of a wild-type and a phoP-null mutant was performed using sam...
ORGANISM(S): Streptomyces coelicolor 
2012-08-30 | E-MAXD-51 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation of Streptomyces coelicolor wild type and phP-null mutants during phosphate starvation to determine binding locations of PhoP
S. coelicolor was grown in low-phosphate concentration R5 liquid medium which facilitated the transition to a phosphate starvation induced stationary phase and the subsequent induction of the PhoP regulon. The genome wide binding locations of PhoP during phosphate starvation were determined by chrom...
ORGANISM(S): Streptomyces coelicolor 
2012-08-30 | E-MAXD-50 | biostudies-arrayexpress
TranscriptomicsEffect of ultradian light-dark cycles on the transcriptome of mice carying a human Per3 polymorphism
In humans, a primate-specific variable-number tandem-repeat (VNTR) polymorphism (4 or 5 repeats 54 nt in length) in the circadian gene PER3 is associated with differences in sleep timing and homeostatic responses to sleep loss. We investigated the effects of this polymorphism on circadian rhythmicit...
ORGANISM(S): Mus musculus 
2014-03-11 | E-GEOD-55495 | biostudies-arrayexpress
TranscriptomicsEffect of 12 hour sleep restriction on the transcriptome of mice carying a human Per3 polymorphism
In humans, a primate-specific variable-number tandem-repeat (VNTR) polymorphism (4 or 5 repeats 54 nt in length) in the circadian gene PER3 is associated with differences in sleep timing and homeostatic responses to sleep loss. We investigated the effects of this polymorphism on circadian rhythmicit...
ORGANISM(S): Mus musculus 
2014-03-11 | E-GEOD-55494 | biostudies-arrayexpress
TranscriptomicsStreptomyces coelicolor cells during various developmental stages: wildtype M145 vs. ΔSCO0204.
Global transcriptional profiling of the SCO0204 null mutant of Streptomyces coelicolor M145 in comparison to the wildtype M145. Goal was to determine the role of SCO0204.
ORGANISM(S): Streptomyces coelicolor A3(2) 
2016-04-14 | E-MTAB-4597 | biostudies-arrayexpress
TranscriptomicsChIP on chip data for AbrC3 binding sites in S. coelicolor M145
ChIP on chip assay of AbrC3 to determine its binding sites in S. coelicolor M145 genome using purified anti-AbrC3 polyclonal antibodies. Samples from nutrient broth (NB) cultures were collected at 36, 48 and 60 hours, cross-linked, and finally hybridized to DNA 104K microarrays designed by Universit...
ORGANISM(S): Streptomyces coelicolor A3(2) 
2014-01-04 | E-MTAB-1451 | biostudies-arrayexpress
TranscriptomicsDifferential expression of S. coelicolor M145 vs. an abrC3 mutant in NB
Differential expression data set of S. coelicolor M145 and a abrC3 mutant cultured in NB. RNA samples were collected at 36, 48 and 60 hours following cDNA synthesis and labeling. The hybridization was conducted using DNA 104K microarrays (Surrey) using gDNA from S. coelicolor M145 as reference.
ORGANISM(S): Streptomyces coelicolor A3(2) 
2014-01-14 | E-MTAB-1453 | biostudies-arrayexpress
TranscriptomicsIdentification of developmentally regulated genes involved in Streptomyces coelicolor sporulation
The aim of this study was to expand our knowledge about the gene repertoire involved in S. coelicolor sporulation. Here we compare the global transcription patterns of the wildtype (M145) parent and two mutants lacking key regulators of aerial hyphal sporulation (whiA and whiH).
ORGANISM(S): Streptomyces coelicolor A3(2) 
2014-01-31 | E-MTAB-1942 | biostudies-arrayexpress
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