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TranscriptomicsIdentifying crossover locations in Arabidopsis thaliana wild-type Col x Ler F2 populations using genotyping by sequencing
Genomic DNA from 74 wild type Col x Ler F2 individuals was extracted using CTAB and used to generate sequencing libraries as described (Rowan et al; Yelina et al), with the following modifications. DNA was extracted from 3 rosette leaves of 5 week old plants and 150 ng of DNA used as input for each ...
ORGANISM(S): Arabidopsis thaliana 
2018-03-17 | E-MTAB-6577 | biostudies-arrayexpress
TranscriptomicsRNA-Seq of human fetal hearts at 9, 12 and 16 weeks of gestation
RNA-Seq was used to study alterations in gene expression in the developing human heart across three gestational ages in the first and early second trimester. All tissue was acquired following elective termination of pregnancy and informed parental consent. Storage and use of tissue was in premises l...
ORGANISM(S): Homo sapiens 
2018-10-24 | E-MTAB-7031 | biostudies-arrayexpress
MetabolomicsSpecific decorations of 17-hydroxygeranyllinalool diterpene glycosides solve the autotoxicity problem of chemical defense in Nicotiana attenuata
The native diploid tobacco Nicotiana attenuata produces abundant, potent anti-herbivore defense metabolites known as 17-hydroxygeranyllinalool diterpene glycosides (HGL-DTGs) whose glycosylation and malonylation biosynthetic steps are regulated by jasmonate signaling. To characterize the biosyntheti...
2021-10-06 | MTBLS1819 | MetaboLights
ProteomicsMembrane Proteomics Reveal Contrasting Adaptation Strategies For Marine Cyanobacteria
Shotgun proteomics focusing on the membrane proteomes, of four Synechococcus spp. strains namely CC9311 (clade I), CC9605 (Clade II), WH8102 (clade III) and CC9902 (clade IV) were conducted to gain insight into the amount of resources these unicellular organisms invest into adaptation strategies.
ORGANISM(S): Synechococcus sp. 
2022-08-30 | PXD009805 | Pride
Transporter Cyanobacteria Membrane 
TranscriptomicsIdentifying crossover locations in an Arabidopsis thaliana Col x Ler F2 population using genotyping by sequencing.
Genomic DNA from 192 Col x Ler F2 individuals was extracted using CTAB and used to generate sequencing libraries as described (Rowan et al; Yelina et al), with the following modifications. DNA was extracted from 3 rosette leaves of 5 week old plants and 150 ng of DNA used as input for each library. ...
ORGANISM(S): Arabidopsis thaliana 
2016-06-11 | E-MTAB-4657 | biostudies-arrayexpress
TranscriptomicsIdentifying crossover locations in an Arabidopsis thaliana meiMIGS-PPX1-PPX2 Col x Ler F2 population using genotyping by sequencing
Genomic DNA from 96 meiMIGS-PPX1-PPX2 Col x Ler F2 individuals was extracted using CTAB and used to generate sequencing libraries as described (Rowan et al; Yelina et al), with the following modifications. DNA was extracted from 3 rosette leaves of 5 week old plants and 150 ng of DNA used as input f...
ORGANISM(S): Arabidopsis thaliana 
2020-12-14 | E-MTAB-9621 | biostudies-arrayexpress
TranscriptomicsIdentifying crossover locations in an Arabidopsis thaliana HEI10 Col x Ler F2 population using genotyping by sequencing.
Genomic DNA from 192 HEI10 Col x Ler F2 individuals was extracted using CTAB and used to generate sequencing libraries as described (Rowan et al; Yelina et al), with the following modifications. DNA was extracted from 3 rosette leaves of 5 week old plants and 150 ng of DNA used as input for each lib...
ORGANISM(S): Arabidopsis thaliana 
2016-08-05 | E-MTAB-4967 | biostudies-arrayexpress
TranscriptomicsIdentifying crossover locations in Arabidopsis thaliana CMT3-11 (Col) x CMT3-7 (Ler) F2 and wild type Col x Ler F2 populations using genotyping by sequencing
Genomic DNA from 384 CMT3-11 (Col) x CMT3-7 (Ler) F2 and 171 wild type Col x Ler F2 individuals was extracted using CTAB and used to generate sequencing libraries as described (Rowan et al; Yelina et al), with the following modifications. DNA was extracted from 3 rosette leaves of 5 week old plants ...
ORGANISM(S): Arabidopsis thaliana 
2017-06-05 | E-MTAB-5476 | biostudies-arrayexpress
TranscriptomicsIdentifying crossover locations in Arabidopsis thaliana wild type Col x CLC, msh2 Col x CLC and msh2 Col x Ler F2 populations using genotyping by sequencing.
Genomic DNA from 189 wild type Col x CLC, 191 msh2 Col x CLC or 187 msh2 Col x Ler F2 individuals was extracted using CTAB and used to generate sequencing libraries as described (Serra et al 2018 PNAS). Sequencing data was analysed to identify crossovers as previously reported, using the TIGER pipel...
ORGANISM(S): Arabidopsis thaliana 
2020-09-02 | E-MTAB-8252 | biostudies-arrayexpress
TranscriptomicsIdentifying crossover locations in Arabidopsis thaliana wild type and taf4b Col × Bur F2 populations using genotyping-by-sequencing
Genomic DNA from 180 Col/Bur F2 individuals, and 186 taf4b/Bur F2 individuals, was extracted by CTAB and used to generate sequencing libraries as previously described (Ziolkowski et al, 2017 Genes & Dev). Sequencing data was analysed to identify crossovers using the TIGER pipeline as previously desc...
ORGANISM(S): Arabidopsis thaliana 
2019-12-31 | E-MTAB-8099 | biostudies-arrayexpress
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