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TranscriptomicsMolecular Characterization of Laser Microdissected Circumventricular Organs and Third Ventricle Ependyma in the Adult Rat by Microarray Analysis.
We used LCM and microarrays to analyze the transcriptomes of three CVOs, the SCO, the subfornical organ (SFO), and the PG, and the third ventricle ependyma in the adult rat in order to better characterize these organs at the molecular level. Gene expression was performed on laser microdissection of...
ORGANISM(S): Rattus norvegicus 
2012-05-23 | E-GEOD-35089 | biostudies-arrayexpress
TranscriptomicsExpression data from LCM captured prostate cancer cells
The prostate represents a complex mix of cell types and there is a need to analyze distinct cell populations to better understand their potential interactions. This study of cell-type specific gene expression patterns will contribute to understanding of how tumor epithelial cells may be affected by...
ORGANISM(S): Homo sapiens 
2010-04-10 | E-GEOD-20758 | biostudies-arrayexpress
TranscriptomicsGene Expression Data from Senescent vs Non-senescent Cells in the in Vivo Human Annulus
The presence of senescent cells in the aging/degenerating human disc is now well-recognized. Senescent cells are viable, cannot divide, remain metabolically active and accumulate within the disc over time. Molecular analysis of senescent cells in tissue, however, offers a special challenge since the...
ORGANISM(S): Homo sapiens 
2009-07-15 | E-GEOD-17077 | biostudies-arrayexpress
TranscriptomicsLaser-capture microscopy coupled with Smart-seq2 (LCM-seq) for robust and efficient transcriptomic profiling of mouse and human tissues
Generally, when LCM is used in diverse transcriptomic analyses, several hundred, if not thousands, of cells are needed to obtain high quality of RNA-seq data. As some cellular populations are very small and tissue often in scarcity, we aimed to carefully document the lowest number of cells needed to...
ORGANISM(S): Homo sapiens 
2016-07-05 | E-GEOD-76514 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse periglomerular cells from laser microdissection (LMD) samples
Analysis of expression of periglomerular cells from laser microdissection (LMD) samples
ORGANISM(S): Mus musculus 
2007-11-10 | E-MEXP-1234 | biostudies-arrayexpress
ProteomicsOne-STAGE Tip Method for TMT based Proteomic Analysis of Minimal Amount of Cells
LC-MS-based profiling of proteomes with isobaric tag labeling from low-quantity biological and clinical samples, including needle-core biopsies and laser capture microdissection, has been challenging due to limited amount and sample loss during preparation. To address this problem, we developed OnM ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-04-18 | MSV000091734 | MassIVE
TranscriptomicsFiber and NMJ / synaptic gene expression comparisons in rat extraocular muscle (EOM) and tibialis anterior (TA) muscle.
Purpose: To examine and characterize the expression profile of genes expressed at the neuromuscular junctions (NMJs) of extraocular muscles (EOMs) in comparison to the NMJs of tibialis anterior muscle (TA). Methods: Adult rat rectus EOMs and TAs were dissected, flash-frozen, serially sectioned and ...
ORGANISM(S): Rattus norvegicus 
2010-04-28 | E-GEOD-15737 | biostudies-arrayexpress
ProteomicsProteomic analysis of laser-microdissected murine fetal glomeruli
Alteration of fetal kidney morphogenesis in diabetic pregnancies is poorly described, especially changes in the extracellular matrix (ECM) and glomerular basement membrane (GBM) during glomerulogenesis. Addressing the ECM proteome, or matrisome, in mouse fetal glomeruli in a healthy and diabetic env...
ORGANISM(S): Mus musculus (Mouse) 
2021-10-13 | PXD025911 | Pride
Mouse Tandem mass spectrometry Glomerulus Laser microdissection microscopy Glomerular basement membrane Extracellular matrix 
UnknownEffect of laser suture lysis on filtration openings: a prospective three-dimensional anterior segment optical coherence tomography study.
Not available
S-EPMC4565948 | biostudies-literature
TranscriptomicsExpression profiling of lesional and non-lesional bulge cells from Lichen planopilaris patients
Lichen planopilaris (LPP) is a chronic inflammatory disease of unknown pathogenesis that leads to permanent hair loss. Whilst destruction of epithelial hair follicle stem cells (eHFSCs) that reside in an immunologically protected niche of the HF epithelium, the bulge, is a likely key event in LPP pa...
ORGANISM(S): Homo sapiens 
2013-07-04 | E-MEXP-3859 | biostudies-arrayexpress
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