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ProteomicsRNA polymerase II Immunoprecipitation from Arabidopsis LC-MSMS
RNA polymerase II (Pol II) was immunoprecipitated from Arabidopsis thaliana seedlings, using antibodies that specifically recognize: 1) C’ terminal Domain (CTD), 2) CTD Serine 5 Phosphorylation and 3) CTD Serine 2 Phosphorylation. Proteins that co-immunoprecipitate with different Pol II pools were ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-10-17 | PXD006004 | Pride
Arabidopsis Lc-msms Pol ii 
ProteomicsAnalysis of the splicing factor LUC7 in Arabidopsis
Characterization of an U1 snRNP component (LUC7) in plants through transcriptome analysis of luc7 Arabidopsis mutant and AP-MS for detecting LUC7 interacting partners
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-02-22 | PXD006127 | Pride
U1 snrnp Arabidopsis Splicing Plants Luc7 
ProteomicsIdentification of U1 snRNA associated proteins
In this project, we isolate U1 snRNA associated proteins in Arabidopsis thaliana. We used an antisense oligonucleotide specific for the U1 snRNA and analyzed associated proteins by mass spectrometry. As a control, the same experiments were performed with U2 snRNA- and lacZ-specifc antisense oligonuc...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-12-04 | PXD045484 | Pride
U1 snrna Arabidopsis U2 snrna Comprehensive isolation by rna binding proteins (chirp) 
TranscriptomicsGlobal effects of the small RNA biogenesis machinery on the Arabidopsis thaliana transcriptome
In Arabidopsis thaliana, four different DICER-LIKE (DCL) proteins have distinct, but partially overlapping functions in the biogenesis of microRNAs (miRNAs) and small interfering RNAs (siRNAs) from longer, non-coding precursor RNAs. To analyze the impact of different components of the small RNA (...
ORGANISM(S): Arabidopsis thaliana 
2010-05-07 | E-GEOD-21685 | biostudies-arrayexpress
TranscriptomicsProfiling of total and AGO1-associated small RNAs in Col-0 wild type, rns2-2 and rns2-3 flowers
Sequencing of small RNA libraries prepared from total RNA or RNA extracted from immuno affinity purified AGO1 from Col-0, rns2-2 and rns2-3 flowers. The aim of this experiment was to compare profiles of small RNA expression between Col-0 (wild type) and mutants of the ribonuclease T2 rns2-2 rns2-3 ...
ORGANISM(S): Arabidopsis thaliana 
2020-01-01 | E-MTAB-8493 | biostudies-arrayexpress
TranscriptomicsMIRNA Gene Evolution in Arabidopsis lyrata and Arabidopsis thaliana: Illumina sequencing
Deep sequencing of small RNA from three closely related brassicaceae A. thaliana, A. lyrata and Capsella rubella was done to systematically analyze the evolution of MIRNA genes and their targets. Small RNA were extracted from total RNA by size fractionation and converted to DNA amplicons by serial a...
ORGANISM(S): Arabidopsis lyrata 
2010-05-14 | E-GEOD-20660 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation of Arabidopsis 4 day-old light-treated seedlings using anti H3, CT, pan, anti H3K9me2 and IgG antibodies
ChIP was performed using 100 mg of 4 day-old light-treated seedlings. The following antibodies were used: anti H3, CT, pan (Millipore 07-690), anti H3K9me2 (Diagenode pAb-060-050) and IgG (Sigma I50006).
ORGANISM(S): Arabidopsis thaliana 
2009-12-08 | E-MEXP-2480 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Arabidopsis etiolated seedlings, light-treated seedlings, leaves and flowers hybridized to ATH1 and AGRONOMICS1 arrays
RNA from etiolated seedlings, light-treated seedlings, leaves and flowers was hybridized to ATH1 and AGRONOMICS1 arrays.
ORGANISM(S): Arabidopsis thaliana 
2010-01-01 | E-MEXP-2472 | biostudies-arrayexpress
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