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uSTAT transcriptional program HPC7 cells were fixed with either 1% formaldehyde for 10 mins. Chromatin was isolated, sonicated for 7 mins (30 sec on, 30 sec off), and specific antibodies were used to pull down the transcription factors of insterest after a pre-clearing step. Chromatin was washed, de...
ORGANISM(S): Mus musculus 
This study aimed at the identification of minimally invasive stress biomarkers in the skin mucus of fish submitted to distinct intensities (mild and most intense) of different types of stress i.e., overcrowding (OC), repeated net-handling (NET) and hypoxia (HYP). Fish trials were conducted in tripli...
ORGANISM(S): Sparus aurata 
2023-04-20 | PXD038712 | Pride
In order to unravel the molecular processes involved in the nature of the root peridermis tissue, as well as in the suberin biosynthesis pathways occurring in this tissue, we have made use of Translating Ribosome Affinity Purification (TRAP) followed by RNA sequencing (TRAP-SEQ) on two marker lines;...
ORGANISM(S): Arabidopsis thaliana 
differential miRNA expression between breast tumor tissue and normal tissue 50 tumor samples were analyzed. As control samples, 3 pools of normal tissue (10 normal samples for each pool ) were used.
ORGANISM(S): Homo sapiens 
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