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MetabolomicsFlow cytometry has a significant impact on the cellular metabolome (GC-MS assay)
The characterization of specialized cell subpopulations in a heterogeneous tissue is essential for understanding organ function in health and disease. A popular method of cell isolation is fluorescence-activated cell sorting (FACS) based on probes that bind surface or intracellular markers. In this ...
2018-11-06 | MTBLS634 | MetaboLights
ProteomicsCombined Antibody/Lectin-Enrichment Identifies Extensive Changes in the O-GlcNAc Subproteome Upon Oxidative Stress
O-GlcNAc is thought to regulate proteins in a mannaer analogous to other PTMs, modulating cellular functions including the cellular stress response. The aim of this study was to identify specific cellular networks and protein complexes that are differentially O-GlcNAcylated and/or expressed upon acu...
ORGANISM(S): Mus musculus (Mouse) 
2016-09-29 | PXD000849 | Pride
Oxidative stress Affinity chromatography Silac O-glcnac 
MetabolomicsEffects of coronatine elicitation on growth and metabolic profiles of Lemna paucicostata culture
In this study, the effects of coronatine treatment on the growth, comprehensive metabolic profiles, and productivity of bioactive compounds, including phenolics and phytosterols, in whole plant cultures of Lemna paucicostata were investigated using gas chromatography-mass spectrometry (GC-MS) couple...
2018-09-11 | MTBLS526 | MetaboLights
MetabolomicsA statistical analysis of the effects of urease pre-treatment on the measurement of the urinary metabolome by gas chromatography–mass spectrometry
Urease pre-treatment of urine has been utilized since the early 1960s to remove high levels of urea from samples prior to further processing and analysis by gas chromatography–mass spectrometry (GC–MS). Aside from the obvious depletion or elimination of urea, the effect, if any, of urease pre-treatm...
2014-05-21 | MTBLS71 | MetaboLights
MetabolomicsThe effects of different rootstocks on aroma components, activities and genes expression of aroma-related enzymes in oriental melon fruit

Grafting is widely applied in the cultivation of melon. In this study, 'Qinmi No.1' [Cucumis melo L. (QG)] and 'Ribenxuesong' [Cucurbita maxima Duch. (RG)] were used as rootstocks for 'QingxinYangjiaocui' (Cucumis melo L.). The results showed that grafting with...

2023-11-21 | MTBLS8677 | MetaboLights
MetabolomicsBased on the Metabolomic Approach the Energy Metabolism Responses of Oriental River Prawn Macrobrachium nipponense Hepatopancreas to Acute Hypoxia and Reoxygenation
Hypoxia represents a major physiological challenge for prawns and is a problem in aquaculture. Therefore, an understanding of the metabolic response mechanism of economically important prawn species to hypoxia and re-oxygenation is essential. However, little is known about the intrinsic mechanisms b...
2021-05-17 | MTBLS584 | MetaboLights
MetabolomicsDirect on-swab metabolic profiling of vaginal microbiome:host interactions during pregnancy and preterm birth

The pregnancy vaginal microbiome contributes to risk of preterm birth, the primary cause of death in children under 5 years of age. Here we describe direct on-swab metabolic profiling by Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) for sample preparation-free characterisation of...

2021-09-23 | MTBLS717 | MetaboLights
ProteomicsGATAD2B O-GlcNAcylation regulates breast cancer stem-like potential and drug resistance
The growth of breast tumors is driven and controlled by a subpopulation of cancer cells resembling adult stem cells, which are called cancer stem-like cells (CSCs). In breast cancer, the function and maintenance of CSCs are influenced by protein O-GlcNAcylation and the enzyme responsible for this po...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD058744 | Pride
Cancer stem cell Cancer Nurd Gatad2b : ogt O-glcnac Signaling Chemoresistance 
MetabolomicsEffect of Zanthoxylum bungeanum essential oil on rumen enzyme activity, microbiome, and metabolites in lambs

Antibiotics were once used in animal production to improve productivity and resistance to pathogenic microbiota. However, due to its negative effects, the search for a new class of substances that can replace its efficacy has become one of the urgent problems to be solved. Plant essential oils (E...

2022-09-13 | MTBLS3683 | MetaboLights
TranscriptomicsGlobal effect of RpoS on gene expression in pathogenic Escherichia coli O157:H7 strain EDL933
RpoS is a conserved stress regulator that plays a critical role in survival under stress conditions in Escherichia coli and other γ-proteobacteria. RpoS is also involved in virulence of many pathogens including Salmonella and Vibrio species. Though well characterized in non-pathogenic E. coli K12 st...
ORGANISM(S): Escherichia coli O157:H7 EDL933 
2009-08-03 | E-GEOD-17420 | biostudies-arrayexpress
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