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ProteomicsXenogeneic-free culture of human intestinal stem cells on functional polymer-coated substrates for scalable, clinical-grade stem cell therapy
Inevitable need for basement membrane extract (BME) with undefined constituents, such as Matrigel, for intestinal stem cell (ISC) culture in traditional system poses a significant barrier that must be overcome for the development of clinical-grade, scalable, ready-to-use ISCs. Here, we propose a fun...
ORGANISM(S): Homo sapiens (Human) 
2024-10-22 | PXD050747 | Pride
Intestinal tissue repair Intestinal stem cell derived from 3d human intestinal organoids (isc3d-hio) Stock banking system Xenogeneic-free culture Functional polymer coating 
GenomicsThe University of Hong Kong Colon Cancer Organoids Genomics Study Data Access Committee
Data Access Committee EGAC00001001405
EGAC00001001405 | EGA
GenomicsOrganoid cultures of early-onset colorectal cancers reveal distinct and rare genetic profiles
Sporadic early-onset colorectal cancer (EOCRC) has bad prognosis, yet is poorly represented by cell line models. We examine the key mutational and transcriptomic alterations in an organoid biobank enriched in EOCRCs. We established paired cancer (n=32) and normal organoids (n=18) from 20 patients en...
EGAS00001004063 | EGA
GenomicsWdr5 mediates self-renewal and reprogramming via the embryonic stem cell core transcriptional network
The embryonic stem (ES) cell transcriptional and epigenetic networks are critical for the maintenance of ES cell self-renewal. However, it remains unclear whether components of these networks functionally interact and if so, what factors mediate such interactions. Here we show that WD-repeat protein...
ORGANISM(S): Mus musculus 
2011-04-08 | E-GEOD-22934 | biostudies-arrayexpress
UnknownFlexible Cu2ZnSn(S,Se)4 solar cells with over 10% efficiency and methods of enlarging the cell area.
Not available
S-EPMC6609618 | biostudies-literature
Genomicsena-DATASET-THE UNIVERSITY OF HONG KONG DEPARTMENT OF PATHOLOGY AND PFIZER INC.-18-12-2019-11:12:21:554-1297 - samples
Four micrograms of total RNA was used for cDNA library construction using the KAPA Stranded mRNA-Seq Kit (KR0960-v3.15), following manufacturer's protocol. The adaptor-ligated libraries were enriched by 6 cycles of polymerase chain reaction (PCR). Libraries were sequenced using the Novaseq 6000 with...
EGAD00001005753 | EGA
Genomicsena-DATASET-THE UNIVERSITY OF HONG KONG DEPARTMENT OF PATHOLOGY AND PFIZER INC.-20-12-2019-08:50:56:915-1359 - samples
Five hundred nanograms of genomic DNA was fragmented by Covaris S2, the fragmented DNAs were performed end-repair, A-tailing at the 3 prime end, adaptors ligation with an IDT dual-indexed UMI adaptor system at the terminal ends. The adapter ligated library with size range 300-750bp were selected by...
EGAD00001005759 | EGA
Genomicsena-DATASET-THE UNIVERSITY OF HONG KONG DEPARTMENT OF PATHOLOGY AND PFIZER INC.-18-12-2019-11:18:36:686-1298 - samples
Five hundred fifty nanograms of genomic DNA were input for library preparation after fragmentation by Covaris S2, following the KAPA Hyper Prep Kit (KR0961-V1.14) protocols, with selection for a library size range of 250-450 bp. Three hundred nanograms per library DNA each from 12 samples were norma...
EGAD00001005754 | EGA
UnknownThe Taiwan Precision Medicine Initiative provides a cohort for large-scale studies.
Not available
S-EPMC12675286 | biostudies-literature
UnknownManagement of severe sepsis in patients admitted to Asian intensive care units: prospective cohort study.
Not available
S-EPMC3113333 | biostudies-literature
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