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ProteomicsProteome profiling of Campylobacter jejuni 81-176 at 37°C and 42°C by label-free mass spectrometry
Background: The main natural reservoir for Campylobacter jejuni is the intestinal tract of birds, especially in the context of human nutrition chickens and turkeys play the main role. There C. jejuni multiplies optimally at 42°C, the avian body temperature, while after infection of humans by peroral...
ORGANISM(S): Campylobacter jejuni 
2024-06-16 | PXD029494 | Pride
Mass spectrometry Temperature adaptation Data-independent acquisition Proteome Campylobacter jejuni 
ProteomicsSWATH-LC/MS/MS of the proteomic response in Campylobacter jejuni 81-176 to sublethal concentrations of seven different bile acids
To characterize the adaptation processes of C. jejuni to sublethal concentrations of bile acids, that are a crucial component of the intestinal antimicrobial defense, including cholic- , chenodeoxycholic-, taurocholic-, glycocholic-, deoxycholic-, lithocholic-, and ursodeoxycholic acid, we used prot...
ORGANISM(S): Campylobacter jejuni subsp. jejuni 81-176 
2018-10-01 | PXD009088 | Pride
Swath Bile acids Campylobacter 
ProteomicsUnbiased quantitative analysis of the cardiac phosphoproteome in response to acute β-adrenergic receptor stimulation in vivo
We utilised Super-SILAC mouse technology to perform an unbiased, quantitative analysis of the cardiac phosphoproteome in mice acutely treated in vivo with either saline or isoprenaline (ISO), a non-selective β-AR agonist. Using a customized mass spectrometry-based workflow (Figure 1), our study iden...
ORGANISM(S): Mus musculus (Mouse) 
2021-12-30 | PXD025569 | Pride
Phosphoproteome  mass spectrometry  β-adrenergic receptor  silac 
ProteomicsProteomic proximity mapping by rapamycin-dependent targeting of APEX2
Enhanced ascorbate peroxidase 2 (APEX2) can be used as a genetic tag that produces short-lived yet highly reactive biotin species, allowing the modification of proteins that interact with or are in very close proximity to the tagged protein, ideally within a confined compartment. Biotinylated protei...
ORGANISM(S): Homo sapiens (Human) 
2020-04-15 | PXD009783 | Pride
Apex2 Mass spectrometry Proximity labeling Silac Apex 
ProteomicsSequestosome 1 is part of the interaction network of VAPB
VAPB (Vesicle-Associated-membrane Protein-associated proteins B) is a tail-anchored mem-brane protein of the endoplasmic reticulum that can also be detected at the inner nuclear mem-brane. As a component of many contact sites between the endoplasmic reticulum and other or-ganelles, VAPB is engaged i...
ORGANISM(S): Homo sapiens (Human) 
2022-02-15 | PXD029113 | Pride
Vapb  amyotrophic lateral sclerosis  sqstm1  sequestosome P62 
ProteomicsIdentification of drivers of breast cancer invasion by secretome analysis: insight into CTGF signaling
An altered consistency of tumor microenvironment facilitates the progression of the tumor towards metastasis. Here we combine data from secretome and proteome analysis using mass spectrometry with microarray data from mesenchymal transformed breast cancer cells (MCF-7-EMT) to elucidate the drivers o...
ORGANISM(S): Homo sapiens (Human) 
2020-10-22 | PXD021539 | Pride
Breast cancer Signaling Secretome 
ProteomicsCharacterization of Campylobacter jejuni proteome profiles in co-incubation scenarios
This study aimed to reveal the influence of co-incubation with Enterococcus faecalis, Enterococcus faecium, or Staphylococcus aureus on the proteome of C. jejuni 81–176 using data-independent-acquisition mass spectrometry (DIA-MS). We compared the proteome profiles during co-incubation with the prot...
ORGANISM(S): Campylobacter jejuni subsp. jejuni 81-176 
2023-11-02 | PXD046477 | Pride
Co-incubation Proteomics Staphylococcus aureus Enterococcus faecalis Bile acids Campylobacter jejuni Enterococcus faecium 
ProteomicsThe ceramide-dependent EV secretome differentially affects prostate cancer cell migration
Tumor-derived extracellular vesicles (EVs) play an important role in cancer pro-gression. Neutral Sphingomyelinases (nSMases) are lipid modifying enzymes that modu-late the secretion of EVs from cells. How nSMase activity and therefore ceramide genera-tion affects the composition and functionality o...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD062469 | Pride
Neutral sphingomyelinases Smpd2 Smpd3 Cell migration Extracellular matrix 
ProteomicsA Multiplexed Parallel Reaction Monitoring Assay to monitor bovine Pregnancy-Associated Glycoproteins throughout pregnancy and after gestation - PRM Data
Bovine pregnancy-associated glycoproteins (boPAGs) are extensively glycosylated secretory proteins of trophoblast cells. Roughly 20 different boPAG members are known but their distribution patterns and degree of glycosylation during pregnancy are not well characterized. The objective of the present ...
ORGANISM(S): Bos taurus (Bovine) 
2022-07-05 | PXD034108 | Pride
Parallel reaction monitoring Pregnancy Cattle 
ProteomicsThe response of Haloferax volcanii to salt and temperature stress: a proteome study by label-free mass spectrometry
We performed an in-depth proteome analysis of the haloarchaeal model organism Haloferax volcanii under standard, low and high salt and low and high temperature conditions using label-free mass spectrometry. Qualitative analysis of protein identification data from high pH/reversed phase fractionated ...
ORGANISM(S): Haloferax volcanii DS2 
2019-09-04 | PXD011056 | Pride
Swath Haloferax volcanii Mass spectrometry Stress response Archaea Proteome 
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