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GenomicsGenome-wide maps of Runx3 bound regions in splenic NK cells
ChIP-seq was conducted using freshly isolated (resting) splenic WT NK cells with anti-Runx3 antibody (Ab), anti-H3K4me1 Ab and non-immune serum (NIS) as control. Runx3 and H3K4me1 IP from splenic NK cells isolated by negative selection using NK cell isolation kit (R&D) followed by sorting of NKp46+ ...
ORGANISM(S): Mus musculus 
2013-11-18 | E-GEOD-50127 | biostudies-arrayexpress
GenomicsGenome-wide maps of Runx3 bound regions in IL-2-activated splenic NK cells
ChIP-seq was conducted using splenic WT NK cells cultured for 7 days with IL-2 using anti-Runx3 antibodies (Ab), anti-H3K4me1 Ab and non-immune serum (NIS) as control. Two biological Runx3, one H3K4me1 and two NIS IP repeats from splenic NK cells isolated from individual mice by negative selection u...
ORGANISM(S): Mus musculus 
2013-11-18 | E-GEOD-50129 | biostudies-arrayexpress
GenomicsGenome-wide maps of Runx3 bound regions in splenic NK cells activated in vivo with IL-15
ChIP-seq was conducted using freshly isolated splenic WT NK cells from IL-15/Ra treated mice with anti-Runx3 antibody (Ab) and non-immune serum (NIS) as control. Runx3 and NIS IP from splenic NK cells of IL-15/Ra treated WT mice, isolated by negative selection using NK cell isolation kit (R&D) follo...
ORGANISM(S): Mus musculus 
2014-01-22 | E-GEOD-50126 | biostudies-arrayexpress
TranscriptomicsRunx3 function in splenic NK cells (IL-2 or IL-15)
NK cells are innate immune cells that recognize and kill foreign, virally-infected and tumor cells without the need for prior immunization. NK expansion following viral infection is IL-2 or IL-15-dependent. To identify Runx3 responsive genes, NK cells were isolated from spleen of WT and Runx3-/- mic...
ORGANISM(S): Mus musculus 
2013-11-18 | E-GEOD-50122 | biostudies-arrayexpress
TranscriptomicsRunx3 function in splenic NK cells
NK cells are innate immune cells that recognize and kill foreign, virally-infected and tumor cells without the need for prior immunization. NK expansion following viral infection is IL-2 or IL-15-dependent. To identify Runx3 responsive genes, NK cells were isolated from spleen of WT and Runx3-/- mic...
ORGANISM(S): Mus musculus 
2013-11-18 | E-GEOD-50123 | biostudies-arrayexpress
TranscriptomicsRunx3 function in splenic NK cells activated in vivo by IL-15.
NK cells are innate immune cells that recognize and kill foreign, virally-infected and tumor cells without the need for prior immunization. NK expansion following viral infection is IL-2 or IL-15-dependent. To identify Runx3 regulated genes, NK cells were isolated from spleen of IL15/Ra injected WT...
ORGANISM(S): Mus musculus 
2014-01-22 | E-GEOD-50120 | biostudies-arrayexpress
TranscriptomicsRunx3 function in IL-2-activated splenic CD8+ T cells
CD8+T cells are immune cells that recognize foreign antigens on infected and tumor cells, leading to cytokine-dependent expansion and activation of cytotoxicity towards the targets. To identify Runx3 regulated genes, CD8+ T cells were isolated from spleen of WT and Runx3-/- mice . Six samples (3 WT ...
ORGANISM(S): Mus musculus 
2013-11-18 | E-GEOD-50121 | biostudies-arrayexpress
TranscriptomicsRunx3 function in CD8+ splenic T cells
CD8+T cells are immune cells that recognize foreign antigens on infected and tumor cells, leading to cytokine-dependent expansion and activation of cytotoxicity towards the targets. To identify Runx3 responsive genes, CD8+ T cells were isolated from spleen of WT and Runx3-/- mice . Six samples (3 WT...
ORGANISM(S): Mus musculus 
2013-11-18 | E-GEOD-50119 | biostudies-arrayexpress
GenomicsGenome-wide maps of Runx3 bound regions in splenic IL-2-activated CD8+ T cells
ChIP-seq was conducted on isolated splenic WT CD8+ T cells, TCR-activated and cultured with IL-2 using anti-Runx3 antibodies (Ab), anti-H3K4me1 Ab and non-immune serum (NIS) as control. Two biological Runx3 and two NIS IP repeats from TCR-activated and IL-2 cultured splenic CD8+ T cells isolated by ...
ORGANISM(S): Mus musculus 
2013-11-18 | E-GEOD-50128 | biostudies-arrayexpress
GenomicsGenome-wide maps of Runx3 bound regions in splenic CD8+ T cells.
ChIP-seq was conducted using freshly isolated (resting) splenic WT CD8+ T cells with anti-Runx3 antibody (Ab), anti-H3K4me1 Ab and non-immune serum (NIS) as control. Two biological Runx3 and two NIS IP repeats from splenic CD8+ T cells isolated by positive selection on anti-CD8 magnetic beads, coll...
ORGANISM(S): Mus musculus 
2013-11-18 | E-GEOD-50130 | biostudies-arrayexpress
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