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Proteomicsyeast membrane proteomics using Leu3 labelling
The yeast signal peptide peptidase homologue Ypf1 functionally interacts with the ER-associated degradation (ERAD) factors Dfm1 and Doa10 to control the abundance of the zinc transporter Zrt1. Here we report that Ctr1 is another substrate for this degradation mechanism with deletion of Ypf1 leading ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2016-04-12 | PXD003052 | Pride
Proteomics Erad Leu3 labelling Yeast Membrane 
TranscriptomicsDIP-chip from Cbf1, Leu3, Pho2, Pho4, Rap1, Rox1, and Swi5
DNA Immunoprecipitation was performed using purified, naked, genomic DNA and purified recombinant DNA binding domains for S. cerevisiae transcription factors (Cbf1, Leu3, Pho2, Pho4, Rap1, Rox1, and Swi5) and then competitively hybridized against input DNA on NimbleGen 385k whole-genome, 32bp, tilin...
ORGANISM(S): Saccharomyces cerevisiae 
2010-05-15 | E-GEOD-13751 | biostudies-arrayexpress
TranscriptomicsTime course expression data in wild-type and TF-deletion yeast
To investigate the role of transcriptional factors Gcn4, Leu3, Gat1 and Met31 in the response to 3AT-induced amino acid starvation, we performed time-course microarray studies of wild-type, single deletion and double deletion strains. The analyses provide insight into a complex regulatory response i...
ORGANISM(S): Saccharomyces cerevisiae 
2011-12-31 | E-GEOD-25582 | biostudies-arrayexpress
TranscriptomicsA library of yeast transcription factor motifs
The sequence specificity of DNA-binding proteins is the primary mechanism by which the cell recognizes genomic features. Here, we describe systematic determination of yeast transcription factor DNA-binding specificities. We obtained binding specificities for 112 DNA-binding proteins representing 19 ...
ORGANISM(S): Saccharomyces cerevisiae 
2008-12-26 | E-GEOD-12349 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human memory T cells stimulated with Phl p-extract in healthy and allergic individuals
Differential transcriptional response of memory T cells stimulated with Phl p-extract in healthy and allergic individuals.
ORGANISM(S): Homo sapiens 
2006-01-01 | E-MEXP-243 | biostudies-arrayexpress
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