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ProteomicsCompatibility of distinct label-free proteomic workflows in absolute quantification of proteins linked to the oocyte quality in human follicular fluid (the Triple-Quad TOF workflow)
We present a comprehensive analysis of human follicular fluid proteome by comparing several strategies of sample preparation, mass spectrometry analysis, and data processing, resulting in the identification of 1638 proteins. We devised two separate label-free quantitative workflows, termed after the...
ORGANISM(S): Homo sapiens (Human) 
2021-07-23 | PXD024223 | Pride
Lc-ms/ms Oocyte quality control Human follicular fluid Oocyte maturity Total protein approach Swath-ms Proteome Blastocyst development 
ProteomicsCompatibility of distinct label-free proteomic workflows in absolute quantification of proteins linked to the oocyte quality in human follicular fluid (the Quad-Orbitrap workflow)
We present a comprehensive analysis of human follicular fluid proteome by comparing several strategies of sample preparation, mass spectrometry analysis, and data processing, resulting in the identification of 1638 proteins. We devised two separate label-free quantitative workflows, termed after the...
ORGANISM(S): Homo sapiens (Human) 
2021-07-23 | PXD024347 | Pride
Lc-ms/ms Oocyte quality control Human follicular fluid Oocyte maturity Total protein approach Proteome Blastocyst development 
ProteomicsHuman follicular fluid proteomic and peptidomic composition quantitative studies by SWATH-MS methodology. Applicability of high pH RP-HPLC fractionation
Analysis of proteomic composition of human follicular fluid (hFF) has been proposed as a potential tool of oocyte quality evaluation. In order to develop an efficient method to investigate the hFF proteome and peptidome components, we applied a prefractionation scheme of hFF material consisting of ...
ORGANISM(S): Homo sapiens (Human) 
2018-03-19 | PXD008073 | Pride
Human Follicular fluid Fractionation Swath-ms 
ProteomicsProteome and peptidome of human follicular fluid
Human follicular fluid (hFF) is a natural environment of oocyte maturation, and some components of hFF could be used to judge oocyte capability for fertilization and further development. In our pilot small scale study three samples from four donors (12 samples in total) were analyzed to determine wh...
ORGANISM(S): Homo sapiens (Human) 
2017-07-04 | PXD006550 | Pride
Human Follicular fluid Swath Lc-ms/ms 
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