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ProteomicsMass spectrometry of HCT116 cells
HCT116 cells were transfected with flag-pcDNA3.1 plasmids or flag-MST3 plasmids. Whole-cell extracts were prepared with a lysis buffer, lysed on ice for 10 min, centrifuged at 12,000 rpm for 10 min at 4°C, and the supernatant was collected. The anti-Flag antibody and protein A/G beads were added and...
ORGANISM(S): Homo Sapiens 
2024-03-09 | PXD050513 |
ProteomicsMS analysis of endogenously precipitated DDX20 protein complexes (immunoprecipitation (IP)–MS)
Protein lysates were derived from HEK293T cells and spermatogonial stem cells，and interacting proteins were “pulled-down” for analysis by gel electrophoresis and identification by mass spectrometry. An equal amount of non-specific IgG from rabbit serum (Thermos Fisher Scientific) was used as a negat...
ORGANISM(S): Homo Sapiens Mus Musculus 
2024-04-24 | PXD038171 |
ProteomicsProteomic analysis of the effects of DDX20 deletion in spermatogonia
To investigate the role of DDX20 in spermatogenesis, we generated Ddx20 flox/flox Ddx4-Cre mice to make a germ cell-specific Ddx20 knockout, and isolated spermatogonia from four-day-old mouse testes by THY1 magnetic beads. We then performed proteomic analysis using protein lysates obtained from THY1...
ORGANISM(S): Mus Musculus 
2024-04-24 | PXD038170 |
ProteomicsBCAT1 Confers Resistance to EGFR TKIs through α-KG-dependent Epigenetic Regulation of the Glycolysis Pathway in NSCLC
Epidermal growth factor receptor (EGFR) mutations are leading oncogenic drivers in non-small cell lung cancer (NSCLC). Many third-generation EGFR tyrosine kinase inhibitors (TKIs) have been developed to target EGFR T790M and activating mutations. Osimertinib (AZD9291) was the first approved drug and...
ORGANISM(S): Homo Sapiens 
2023-07-14 | PXD043776 |
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