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TranscriptomicsDetection of Pol IV-dependent transcripts at the genomic scale in Arabidopsis reveals features and regulation of siRNA biogenesis
24 nucleotide siRNAs are central players in RNA-directed DNA methylation (RdDM), a process that establishes DNA methylation at transposable elements to ensure genome stability. The plant-specific RNA polymerase IV (Pol IV) is required for siRNA biogenesis and is thought to transcribe RdDM loci to pr...
ORGANISM(S): Arabidopsis thaliana 
2014-11-11 | E-GEOD-57215 | biostudies-arrayexpress
Proteomics293T NME4 TAP-MS and TurboID-MS
To identify interacting proteins of NME4 in human cell lines, we using tandem affinity purification followed by mass spectrometry (TAP-MS) in HEK293T cells. We established stable cell line which expressing NME4 gene fused with SFB triple tags (S tag-2x Flag tag-SBP tag) or TurboID. We isolated, comb...
ORGANISM(S): Homo sapiens (Human) 
2023-10-13 | PXD036624 | Pride
Tap-ms turboid-ms 
UnknownCluster minimal sufficient balance (CMSB): an efficient covariate balancing randomization method for cluster randomized trials.
Not available
S-EPMC12857073 | biostudies-literature
ProteomicsHuman gastric micropeptides LC-MS/MS
The Human Proteome Project currently seeks to understand the complexities of functional genomics and proteomics and to systematically identify and functionally characterize noncanonical translated sequences, such as micropeptides, will enrich the understanding of the human genome. By producing a hig...
ORGANISM(S): Homo sapiens (Human) 
2025-01-18 | PXD041397 | Pride
Human Gastric cancer Micropeptide 
ProteomicsHuman gastric micropeptide LC-MS/MS
The Human Proteome Project currently seeks to understand the complexities of functional genomics and proteomics and to systematically identify and functionally characterize noncanonical translated sequences, such as micropeptides, will enrich the understanding of the human genome. By producing a hig...
ORGANISM(S): Homo sapiens (Human) 
2025-01-18 | PXD041392 | Pride
Human Micropeptide 
ProteomicsTMT6plex quantitative proteomics of 293T NME4 KO cell
To identify the role NME4,we knockout NME4 in 293T cells. Followed by protein extraction, trypsin digestion, TMT6 labeled, HPLC fractionation and LC-MS/MS.
ORGANISM(S): Homo sapiens (Human) 
2023-10-13 | PXD036628 | Pride
Human nme4 tmt6 
ProteomicsLC-MS/MS Proteomics analysis of PDL1 protein in 293T cell line
To identify the proteins interact with PDL1 protein in human cell lines, we analyzed the protein complexes using tandem affinity purification followed by mass spectrometry (TAP-MS) in HEK293T. We established the cell lines stably expressing PDL1 gene fused with Flag tags. We identified proteins asso...
ORGANISM(S): Homo sapiens (Human) 
2022-10-13 | PXD031702 | Pride
Lc-ms/ms，pdl1，293t 
ProteomicsUrsodeoxycholic acid promoted TGF-β degradation
MS analysis of proteins interacting with PKA and TGF-β1 in HEK293T cells. MS analysis of immunoprecipitated TGF-β1 in HEK293T cells with TGF-β1 overexpression.
ORGANISM(S): Mus musculus (Mouse) 
2022-06-28 | PXD031801 | Pride
Tgf-β1 Pka Phosphorylation modification 
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