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The details of Toll-like receptor (TLR) 4 signaling affects protein succinylation in intracerebral hemorrhage (ICH) brains remains completely unclear. In this study, we constructed mice ICH models to investigate the changes in ICH-associated brain protein succinylation with the treatment of TLR4 ant...
ORGANISM(S): Mus musculus (Mouse) 
2021-11-03 | PXD025622 | Pride
Protein posttranslational modifications (PTMs) has been shown to regulate biological processes of human diseases via expanding the genetic code and for regulating cellular pathophysiology, however, the system-wide changes at the PTMs levels in ICH brain remains little known. Given that succinylation...
ORGANISM(S): Mus musculus (Mouse) 
2021-10-29 | PXD025621 | Pride
To identify the potential circGlis3 binding proteins, we extracted the total protein from MIN6 cells and performed RNA Pull-down assay. Then RNA-interacting proteins were subjected to mass spectrometry.
ORGANISM(S): Mus musculus (Mouse) 
2023-11-02 | PXD037024 | Pride
To identify the novel protein encoded by circGlis3, total protein lysates were extracted from MIN6 cells transfected with circGlis3 plasmid or empty vector and separated by SDS-PAGE, followed by Coomassie brilliant blue staining. The differential gel bands near 35 kDa were excised and subjected to m...
ORGANISM(S): Mus musculus (Mouse) 
2023-11-02 | PXD037447 | Pride
To identify a novel protein circINSIG1-121, we extracted the proteins from HCT8 cells, and then the proteins were subjected to mass spectrometry
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD037292 | Pride
To identify the unknown interacting proteins with circINSIG1-121, we extracted the proteins from HCT8 cells and performed co-immunoprecipitation assays with circINSIG1-121 antibody. Then the proteins were subjected to mass spectrometry
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD037028 | Pride
Recent studies have revealed that long non-coding RNAs (lncRNAs) participate in all steps of cancer initiation and progression by regulating protein coding genes at the epigenetic, transcriptional and post-transcriptional levels. LncRNAs are in turn regulated by other genes, forming a complex regula...
ORGANISM(S): Homo sapiens 
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