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RNA isolation and RNA-Seq: Wild-type Xcc or pXccBphP strains were cultured in far-red light or dark conditions up to logarithmic phase (0.7 to 0.8 OD600) at 28C in PYM broth. Total bacterial RNA was isolated using the MasterPureTM RNA Purification Kit (Epicentre, Illumina). Samples corresponding to ...
ORGANISM(S): Xanthomonas campestris pv. campestris 
Hypoxia-inducible factor 1α (HIF-1α) broadly orchestrates metabolic reprogramming in inflammatory macrophages. However, how HIF-1α is activated in the earliest events following pattern recognition receptor activation remains unclear. We found that HIF-1α is functionally active shortly after macroph...
ORGANISM(S): Mus musculus 
To understand the functions of Pf1 sRNA in response to temperature changes, a knockout mutant (ΔPf1) was constructed. Transcriptomics and proteomics were performed on biological triplicates of the wild strain and ΔPf1 grown to stationary phase at 4°C and 32°C. The file uploaded is the original data ...
ORGANISM(S): Pseudoalteromonas sp. Bsw20308 Bacteria 
2022-12-19 | PXD036967 | Pride
Arsenic (As) is a carcinogenic metalloid that is a contaminant widely polluting rice paddy soils around the world. In order to gain better insight into molecular mechanism of rice exposed to As(III) stress, we used next-generation sequencing technology to acquire global transcriptome alteration and ...
ORGANISM(S): Oryza sativa Japonica Group 
Secreted bacterial RNAs have recently emerged as a novel host-pathogen interaction mode. Naked RNA molecules are highly labile in the extracellular environment and must be protected by packaging into membrane vesicles or into complexes with RNA binding proteins. RNA secretion through membrane vesicl...
ORGANISM(S): Listeria monocytogenes EGD-e 
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