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MetabolomicsMetabolic Profiling of Escherichia coli-Based Cell-Free Expression Systems for Process Optimization
Biotechnology has transformed the production of various chemicals and pharmaceuticals due to its efficient and selective processes, but it is inherently limited by its use of live cells as 'biocatalysts.' Cell-free expression (CFE) systems, which use a protein lysate isolated from whole cells, have ...
2020-06-02 | MTBLS1079 | MetaboLights
MetabolomicsMycelium of Diaporthe nobilis treated with 2% cell-free supernatant (CFS) from Bacillus velezensis A4 for 12 hours

Mycelia of Diaporthe nobilis(50 mg) treated with CFS were collected,Each sample was homogenized in 400 µL of pre-cooled 80% methanol aqueous solution containing 0.02 mg/mL L-2-chlorophenylalanine (internal standard). The mixture was ground and subjected to ultrasonication at 4 °C for 30 min. Afte...

2025-11-19 | MTBLS13351 | MetaboLights
MetabolomicsMetabolomic Sequencing of Cell-Free Supernatant from Bacillus strain 541

The 4-6 minute fraction from the HPLC fractionation of the bacterial culture supernatant of porcine-derived probiotic Bacillus strain 541.

2025-09-08 | MTBLS12956 | MetaboLights
ProteomicsEvaluation of library-free strategies for phosphoproteomics and ubiquitinomics data-independent acquisition data
Phosphoproteomics and ubiquitinomics data-independent acquisition MS data is generally analyzed using a DDA spectral library. Performance of different library-free strategies of analyzing phosphoproteomics and ubiquitinomics DIA MS data are not evaluated. In this study, we assess three library-free ...
ORGANISM(S): Homo Sapiens 
2023-05-17 | PXD037870 |
GenomicsPCR-free library preparation
A method for PCR-free library preparation for sequencing palaeogenomes
PRJEB83706 | ENA
ProteomicsDIA-MS2pep : a library-free framework for comprehensive peptide identifications from DIA-data
We present, a new framework for library-free peptide identification from data-independent acquisition (DIA) data, DIA-MS2pep, containing two main components: 1) MS2 spectrum self-demultiplexing and 2) large precursor mass tolerance database search. DIA-MS2pep empowers the discovery capability of DIA...
ORGANISM(S): Homo Sapiens Escherichia Coli Caenorhabditis Elegans Saccharomyces Cerevisiae 
2022-03-14 | PXD032253 |
MetabolomicsAnalysis of Volatile Organic Compounds (VOCs) in Streptomyces sp. 30177 treated with cell-free filtrate of Sphingomonas sp. 06703 using Headspace Solid-Phase Microextraction (HS-SPME) coupled with Gas Chromatography-Mass Spectrometry (GC-MS)
This study aimed to analyze the volatile organic compounds (VOCs) produced by Streptomyces sp. 30177 in response to treatment with the cell-free filtrate of Sphingomonas sp. 06703. The cell-free filtrate was prepared by culturing Sphingomonas sp. 06703 in liquid medium, followed by centrifugation an...
2026-07-23 | MTBLS14913 | MetaboLights
ProteomicsCavia porcellus spectral library
The guinea pig (cavia porcellus) is an excellent experimental model for translation to many aspects of human physiology and disease yet there is limited experimental information regarding its proteome. In an effort to overcome this gap in our knowledge, we generated a comprehensive spectral library ...
ORGANISM(S): Cavia Porcellus (ncbitaxon:10141) 
2018-12-07 | MSV000083199 | MassIVE
GenomicsSystematic calibration of epitranscriptomic atlas using a synthetic modification-free RNA library
Systematic calibration of epitranscriptomic atlas using a synthetic modification-free RNA library
PRJNA634388 | ENA
ProteomicsCreation of a Plant Metabolite Spectral Library for Untargeted and Targeted Metabolomics
Large-scale high throughput metabolomic technologies are indispensable components of systems biology in terms of discovering and defining the metabolite parts of the system. However, the lack of a plant metabolite spectral library limited the metabolite identification of plant metabolomics study. He...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2022-12-03 | MSV000090829 | MassIVE
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