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The EC strain karyotype was analyzed by array-CGH to identify amplified chromosomal regions. EC9 was chosen as the representative strain of EC-C1 strain
ORGANISM(S): Saccharomyces cerevisiae 
The genomic DNA of wild-type chromosome VIII of evolved EC9-7 cells was extracted from the gel of pulsed field gel electrophoresis (PFGE), and analyzed by array-CGH to identify its chromosomal composition Yeast genomic DNA was extracted using QIAGEN Genomic-tip 100/G kit. After PFGE, The ChrVIII DN...
ORGANISM(S): Saccharomyces cerevisiae 
The genomic DNA of rearranged chromosome VIII (~900kb) of EC9 diploid strain was extracted from the gel of pulsed field gel electrophoresis (PFGE), and analyzed by array-CGH to identify it's amplified regions After PFGE, The ChrVIII DNA was purified from agarose gel and purified by column. Then the...
ORGANISM(S): Saccharomyces cerevisiae 
Since Usp11 encodes a DUB, we reasoned that Usp11 deficiency may lead to increased ubiquitination and destabilization of a protein that functions in neuronal differentiation and migration.To define the molecular mechanism of Usp11 deficiency on cortical development, we performed a label-free quantit...
ORGANISM(S): Mus musculus (Mouse) 
2021-01-29 | PXD021587 | Pride
Using unbiased mass spectrometry-based screen to identify differences in ubiquitinated proteins in the overexpression of Usp11. To focus on potential substrates relevant for Usp11’s role in neurogenesis, we performed experiments with mouse embryonic stem (ES) cells, in which NPC and neurons are deri...
ORGANISM(S): Mus musculus (Mouse) 
2021-01-29 | PXD021189 | Pride
The expression profile of natural isolate of S. cerevisiae carrying different copy of CUP2 were compared under cooper sulfate stress The gene expression profiles of natural isolate of S.cerevisiae(EC9-7), EC9-7 cup2∆ and Ec9-7 cup2∆cup2∆ strains under YPD with 1mM copper sulfate for 1h and rich me...
ORGANISM(S): Saccharomyces cerevisiae 

Irritable bowel syndrome (IBS) is one of the functional gastrointestinal disorders characterized by chronic and/or recurrent symptoms of abdominal pain and irregular defecation. Changed gut microbiota has been proposed to mediate IBS; however, contradictory results exist, and IBS-specific microbi...

2021-12-01 | MTBLS2774 | MetaboLights
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