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A 2D-LC-MS/MS strategy coupled with TMT-10 labeling was applied to mouse pancreatic beta cells (hetaTC-6) induced with ER stress by Thapsigargin to study the global proteome and protein cysteine PTM changes under ER stress. Samples were digested with trypsin, labeled with 10-plex TMT, then analyzed ...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2021-05-29 | MSV000087543 | MassIVE
To increase the coverage of membrane protein identification,we developed high-pH reverse phase (Hp-RP) combined with stop-and-go extraction tip (StageTip) technique, as a fast (∼15 min.). Almost 5000 proteins (∼60% membrane proteins) can be identified in only 10 μg of membrane protein digests, showi...
ORGANISM(S): Homo Sapiens (human) 
Mining Missing Membrane Proteins, Hp-RP StageTips, CL83
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005364 | PeptideAtlas
Mining Missing Membrane Proteins, Hp-RP StageTips, A549
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005358 | PeptideAtlas
Mining Missing Membrane Proteins, Hp-RP StageTips, PC9
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005379 | PeptideAtlas
Mining Missing Membrane Proteins, Hp-RP StageTips, H3255
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005336 | PeptideAtlas
Mining Missing Membrane Proteins, Hp-RP StageTips, CL97
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005449 | PeptideAtlas
Mining Missing Membrane Proteins, Hp-RP StageTips, CL141
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005374 | PeptideAtlas
Mining Missing Membrane Proteins, Hp-RP StageTips, CL100
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005443 | PeptideAtlas
Mining Missing Membrane Proteins, Hp-RP StageTips, CL152
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005416 | PeptideAtlas
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