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TranscriptomicsTempO-Seq of human breast cancer MCF-7 and HCC1143 cell lines under acute normoxia and hypoxia
Comparison of luminal and basal breast cancer cells under acute normoxia and hypoxia. Cells were plated in 96-well plates and incubated 24 hrs under normoxia or hypoxia after which the wells were washed once with cold PBS and lysed using TempO-Seq lysis buffer for 15 min at room temperature. Samples...
ORGANISM(S): Homo sapiens 
2023-06-01 | E-MTAB-12234 | biostudies-arrayexpress
UnknownDendritic Cells Cause Bone Lesions in a New Mouse Model of Histiocytosis.
Not available
S-EPMC4527720 | biostudies-literature
TranscriptomicsTempO-Seq of human breast cancer MCF-7 and HCC1143 cell lines under chronic normoxia and hypoxia
Comparison of luminal and basal breast cancer cells under chronic normoxia and hypoxia. Cells were plated in 96-well plates and incubated 5 days under normoxia or hypoxia after which the wells were washed once with cold PBS and lysed using TempO-Seq lysis buffer for 15 min at room temperature. Sampl...
ORGANISM(S): Homo sapiens 
2023-06-01 | E-MTAB-12233 | biostudies-arrayexpress
TranscriptomicsA global analysis of tandem 3'UTRs in eosinophilic chronic rhinosinusitis with nasal polyps
Background: Alternative polyadenylation (APA) is emerging as a widespread mechanism of gene regulation. The usage of APA sites allows a single gene to encode multiple mRNA transcripts with different 3'-untranslated region (3'UTR) lengths. Many disease processes reflect the importance of the regulati...
ORGANISM(S): Homo sapiens 
2012-12-31 | E-GEOD-39957 | biostudies-arrayexpress
TranscriptomicsM. catarrhalis Gene Expression in the Chinchilla Nasopharynx
M. catarrhalis strain O35E.rpsl was inoculated into the nasopharynx of healthy, male adult chinchillas. 24 hours later the nasopharyngeal tissues were extracted and homogenized. Total RNA was extracted from these tissue samples. Subsequently, M. catarrhalis genome directed primers were utilized t...
ORGANISM(S): Moraxella catarrhalis 
2012-04-03 | E-GEOD-13559 | biostudies-arrayexpress
TranscriptomicsMicroRNA expression in chronic rhinosinusitis
Increasing evidence suggests that CRS is a heterogeneous group of sinus disorders involving overlapping but distinct disease entities.The factors leading to different CRS phenotypes remain enigmatic. The role of miRNAs in the regulation of immunological and inflammatory processes is beginning to eme...
ORGANISM(S): Homo sapiens 
2011-12-08 | E-GEOD-32300 | biostudies-arrayexpress
UnknownGuidelines for the use of flow cytometry and cell sorting in immunological studies.
Not available
S-EPMC9165548 | biostudies-literature
UnknownGuidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).
Not available
S-EPMC7350392 | biostudies-literature
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