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ProteomicsAutomated sample preparation with SP3 for low-input clinical proteomics
High-throughput and streamlined workflows are essential in clinical proteomics for standardized processing of samples originating from a variety of sources, including frozen tissue, FFPE tissue, or blood. To reach this goal, we have implemented single-pot solid-phase-enhanced sample preparation (SP3...
ORGANISM(S): Homo sapiens (Human) 
2020-01-10 | PXD014556 | Pride
Sp3 Clinical proteomics Pulmonary adenocarcinoma Sample preparation Low-input High-throughput Automation 
ProteomicsAutomated sample preparation with SP3 for low-input clinical proteomics
High-throughput and streamlined workflows are essential in clinical proteomics for standardized processing of samples originating from a variety of sources, including frozen tissue, FFPE tissue, or blood. To reach this goal, we have implemented single-pot solid-phase-enhanced sample preparation (SP3...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) Sus scrofa domesticus (domestic pig) 
2020-03-27 | PXD015840 | Pride
Fresh-frozen tissue Proteomics Sp3 Cells Sample preparation 
ProteomicsFully automated sample processing and analysis workflow for low-input proteome profiling
Recent advances in sample preparation and analysis have enabled direct profiling of protein expression in single mammalian cells and other trace samples for characterization of cellular heterogeneity and high-resolution mapping of tissues. Several techniques used to prepare and analyze low-input sam...
ORGANISM(S): Homo sapiens (Human) 
2020-12-21 | PXD021882 | Pride
Low-input sample preparation Low-input proteomics 
ProteomicsLow-input proteomics using lauryl maltose neopentyl glycol
We developed a sample preparation method for low-input proteomics using lauryl maltose neopentyl glycol. Using the developed method, we performed proteome analysis of coIP samples.
ORGANISM(S): Homo Sapiens (human) 
2024-03-06 | PXD048100 | JPOST Repository
ProteomicsLow-input proteomics using lauryl maltose neopentyl glycol
We developed a sample preparation method for low-input proteomics using lauryl maltose neopentyl glycol. Using the developed method, we performed proteome analysis of 100ng of HEK293T proteins, coIP samples, and serum extracellular vesicles, and further challenged label-free single-cell proteomics o...
ORGANISM(S): Homo Sapiens (human) Bos Taurus 
2024-03-06 | PXD044605 | JPOST Repository
GenomicsMicrofluidic platform for next-generation sequencing library preparation with low-input samples
Microfluidic platform for next-generation sequencing library preparation with low-input samples
PRJNA548967 | ENA
ProteomicsSuspension TRAPping filter (sTRAP) sample preparation for quantitative proteomics in the low µg input range using a plasmid DNA micro-spin column: analysis of the hippocampus from the 5xFAD Alzheimer’s Disease mouse model
Suspension TRAPping filter (sTRAP) is an attractive sample preparation method for proteomics study. Here, we demonstrated the use of a low-cost Plasmid DNA spin column for sTRAP. We first evaluated, the reproducibility of this protocol and the low CoV using 4 replicates of 5-10 µg of a synapse enric...
ORGANISM(S): Mus musculus (Mouse) 
2023-05-23 | PXD041045 | Pride
Alzheimer’s disease Proteomics 5xfad mouse model Micro-spin column Strap 
ProteomicsSolvent Precipitation SP3 (SP4) enhances recovery for proteomics sample preparation without magnetic beads
Complete, reproducible extraction of protein material is essential for comprehensive and unbiased proteome analyses. A current gold standard is single-pot, solid-phase-enhanced sample preparation (SP3), in which organic solvent and magnetic beads are used to denature and capture proteins, with subse...
ORGANISM(S): Homo sapiens (Human) 
2022-08-04 | PXD028736 | Pride
Proteomics Solvent precipitation Sample preparation 
GenomicsComparative evaluation of RNA-Seq library preparation methods for strand-specificity and low input
Comparative evaluation of RNA-Seq library preparation methods for strand-specificity and low input
PRJNA510931 | ENA
GenomicsMicrofluidic platform for next-generation sequencing library preparation with low-input samples
Advances in Next Generation Sequencing (NGS) have made available a wealth of information that had previously been inaccessible to researchers and clinicians. NGS has been applied to understand genomic, transcriptomic, and epigenomic changes and gained traction as a significant tool capable of accele...
ORGANISM(S): Homo sapiens Mus musculus 
2020-01-02 | GSE132786 | GEO
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