Objective: This study investigated the therapeutic mechanism by which Xuezhikang (XZK) alleviates non-alcoholic fatty liver disease (NAFLD) in mice, with a specific focus on the interplay between the gut microbiota, metabolic alterations, and hepatic ferroptosis. Methods: An NAFLD mouse model was in...
For MS of BCL6 modification sites and interaction proteins, the gel band of BCL6 was excised and digested with trypsin. Immunocomplexes were identified on Thermo Scientific Orbitrap Fusion Lumos. The data for this project include raw data.
We compared the performance of multidimensional protein identification (MudPIT) on Velos Pro Orbitrap (VPO) and Velos Orbitrap Elite (VOE) mass spectrometers to single dimension reversed phase (RP) chromatography on a Q Exactive Plus (QE+) and an Orbitrap Fusion™ Lumos™ (OFL). Using a digested HeLa...
DHX15 is an ATP-dependent RNA helicase involved in pre-mRNA splicing. We have recently reported that DHX15 is a downstream substrate for Akt1, which plays a significant role in vascular biology. Therefore, we aimed to explore the regulatory function of DHX15 over the vasculature, and the endothelial...
Identification of protein-protein interactions is a major goal of biological research. Despite technical advances over the last two decades, important but still largely unsolved challenges include the high-throughput detection of interactions directly from primary tissue and the identification of in...
Protein lysine residue carbamylation is an irreversible post-translational modification, resulting in the generation of protein-bound homocitrulline (N--carbamyllysine). Carbamylation alters protein structure and can thus also alter protein function because of the loss of the charged -amino lysine m...
We have compared three different glycopeptide enrichment techniques (SAX-ERLIC, HILIC, and lectin affinity) and a C18 control to a tryptic digest of proteins from depleted human plasma.
Translation elongation factor 1A (eEF1A) is an essential and highly conserved protein required for protein synthesis in eukaryotes. In both Saccharomyces cerevisiae and human, five different methyltransferase enzymes methylate specific residues on eEF1A, making eEF1A the eukaryotic protein targeted ...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast)
Foodcrusts are often discovered inside archaeological pottery and other containers. They can provide abundant information about vessel function, diet, cuisine activities and human subsistence strategies. Lipid analysis has been used for decades to characterize foodcrusts, indicating their compositio...
Foodcrusts are often discovered inside archaeological pottery and other containers. They can provide abundant information about vessel function, diet, cuisine activities and human subsistence strategies. Lipid analysis has been used for decades to characterize foodcrusts, indicating their compositio...
