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Metabolomics13C glucose labelling studies using 2D NMR is a useful tool for determining ex vivo whole organ metabolism during hypothermic machine perfusion of kidneys
Background: The aim of this study is to determine the feasibility of using Nuclear Magnetic Resonance (NMR) tracer studies (13C-enriched glucose) to detect ex vivo de novo metabolism in the perfusion fluid and cortical tissue of porcine kidneys during hypothermic machine perfusion (HMP). Methods: Po...
2017-10-16 | MTBLS367 | MetaboLights
MetabolomicsMetabolomics to elucidate the effects of novel sustainable feed additives in the feed of farmed salmon (HoloFood Trial D - fermented seaweed open water-dose response)

Testing the effect of seaweed as a novel cost-efficient feed source for Atlantic salmon feed. Fermentation of novel raw materials for fish feed is a new practice with little prior experience of direct relevance to aquaculture and fish farming. In Trial D, an alternative marine compound is include...

2023-06-13 | MTBLS6733 | MetaboLights
ProteomicsProteomic analysis reveals enzymes for β-D-glucan formation and degradation in Levilactobacillus brevis TMW 1.2112
Bacterial exopolysaccharide (EPS) formation is crucial for biofilm formation, protection against environmental factors or as storage compounds. EPSs produced by lactic acid bacteria (LAB) are appropriate for applications in food fermentation or the pharmaceutical industry, yet the dynam-ics of forma...
ORGANISM(S): Levilactobacillus brevis 
2022-03-21 | PXD031809 | Pride
Moonlighting proteins Glycosyl hydrolase Β-glucan Exopolysaccharide Levilactobacillus brevis tmw 1.2112 Glycosyltransferase Proteome Secretome 
UnknownDo extreme summers increase blood vitamin D (25-hydroxyvitamin D) levels?
Not available
S-EPMC7654803 | biostudies-literature
ProteomicsE-selectin-mediated rapid NLRP3 inflammasome activation regulates S100A8/A9 release from neutrophils via transient gasdermin D pore formation
S100A8/A9 is a proinflammatory mediator released by myeloid cells during many acute and chronic inflammatory disorders. However, the precise mechanism of its release from the cytosolic compartment of neutrophils is still elusive. We report here that E-selectin-induced rapid S100A8/A9 release during ...
ORGANISM(S): Homo sapiens (Human) 
2023-10-25 | PXD041652 | Pride
Neutrophile Inflammation Gasdermin d Transient pore formation 
MetabolomicsMetabolomics reveals an involvement of pantothenate for male production responding to the short-day stimulus in the water flea, Daphnia pulex
Under favorable conditions, the micro-crustacean Daphnia pulex produces female offspring by parthenogenesis, whereas in response to various unfavorable external cues, they produce male offspring to induce sexual reproduction (environmental sex determination: ESD). We recently established a suitable ...
2017-04-03 | MTBLS259 | MetaboLights
ProteomicsFaecal Proteases and Immune Signatures Drive Subtype-Specific Enteric Neuronal Activation in IBS
Background: Luminal proteases have been implicated in epithelial barrier dysfunction and visceral hypersensitivity in irritable bowel syndrome (IBS), yet their impact on the enteric nervous system (ENS), the principal regulator of gastrointestinal function, remains unknown. We investigated whether ...
ORGANISM(S): Homo sapiens (Human) 
2026-09-10 | PXD071836 | Pride
Protease-activated receptors (pars) Irritable bowel syndrome Serine and cysteine protease activity Faecal supernatant proteomics Luminal–neuronal signalling axis 
ProteomicsIdentification of allergenomic signatures in allergic and well-tolerated apple genotypes using LC-MS/MS
The apple fruit (Malus domestica L. Borkh) is one of the most popular fruits worldwide. Beyond their beneficial properties, apples contain proteins that trigger allergic reactions in susceptible consumers. Mal d1 to 4 are allergens present in a variety of different isoforms in apples, with Mal d1 an...
ORGANISM(S): Malus domestica 
2022-05-24 | PXD027753 | Pride
Mal d proteins Proteomics Apple breeding Malus domestica Hypoallergenic Lc-ms/ms 
ProteomicsLong-term exposure of Daphnia magna to polystyrene microplastics induces changes in their proteome.
In the past years, the research focus on the effects of microplastics (MP) on aquatic organisms extended from marine systems towards freshwater systems. An important freshwater model organism in the MP field is the cladoceran Daphnia, which plays a central role in lacustrine ecosystems and has been ...
ORGANISM(S): Daphnia magna 
2021-07-07 | PXD025108 | Pride
Proteomics Polystyrene Mass spectrometry Microplastics Daphnia 
Unknown2-De-oxy-α-d-arabino-hexopyran-ose.
Not available
S-EPMC3201514 | biostudies-literature
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